Measurement of Lipase Activity of Guinea Pig Peritoneal Macrophages with 4-Methylumbelliferyl-oleate

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Title ( eng )
Measurement of Lipase Activity of Guinea Pig Peritoneal Macrophages with 4-Methylumbelliferyl-oleate
Title ( jpn )
4-Methylumbelliferyl-oleate を用いるモルモット腹腔マクロファージのリバーゼ活性測定法
Creator
Kiyotani Katsuhiro
Tasaka Hiromichi
Matsuo Yoshiyasu
Source Title
Hiroshima Journal of Medical Sciences
Volume 32
Issue 1
Start Page 15
End Page 18
Journal Identifire
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
Abstract
Some basic conditions for fluorometric measurement of lipase activity of guinea pig peritoneal macrophages were investigated using 4-methylumbelliferyl-oleate as a substrate. The most adequate condition for the assay is as follows:

i ) Enzyme preparation recommended is the supernatant of ultrasonicate after freezing and thawing of guinea pig peritoneal macrophages suspended in distilled water.

ii) Final concentration of buffered substrate should be adjusted at 0.1 mM in acetate buffer, pH 4.5, free from Triton X-100.

iii) Reaction is terminated by addition of 50 mM Tris-HCl buffer, pH 8.6, instead of 50 mM glycine buffer, pH 10.4.
Keywords
4-Methylumbelliferyl-oleate
Lipase
Triton X-100
NDC
Medical sciences [ 490 ]
Language
eng
Resource Type departmental bulletin paper
Publisher
Hiroshima University School of Medicine
Date of Issued 1983-03
Publish Type Version of Record
Access Rights open access
Source Identifier
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 6688069