Measurement of Lipase Activity of Guinea Pig Peritoneal Macrophages with 4-Methylumbelliferyl-oleate
Hiroshima Journal of Medical Sciences Volume 32 Issue 1
Page 15-18
published_at 1983-03
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Title ( eng ) |
Measurement of Lipase Activity of Guinea Pig Peritoneal Macrophages with 4-Methylumbelliferyl-oleate
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Title ( jpn ) |
4-Methylumbelliferyl-oleate を用いるモルモット腹腔マクロファージのリバーゼ活性測定法
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Creator |
Kiyotani Katsuhiro
Tasaka Hiromichi
Matsuo Yoshiyasu
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Source Title |
Hiroshima Journal of Medical Sciences
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Volume | 32 |
Issue | 1 |
Start Page | 15 |
End Page | 18 |
Journal Identifire |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
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Abstract |
Some basic conditions for fluorometric measurement of lipase activity of guinea pig peritoneal macrophages were investigated using 4-methylumbelliferyl-oleate as a substrate. The most adequate condition for the assay is as follows:
i ) Enzyme preparation recommended is the supernatant of ultrasonicate after freezing and thawing of guinea pig peritoneal macrophages suspended in distilled water. ii) Final concentration of buffered substrate should be adjusted at 0.1 mM in acetate buffer, pH 4.5, free from Triton X-100. iii) Reaction is terminated by addition of 50 mM Tris-HCl buffer, pH 8.6, instead of 50 mM glycine buffer, pH 10.4. |
Keywords |
4-Methylumbelliferyl-oleate
Lipase
Triton X-100
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NDC |
Medical sciences [ 490 ]
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Language |
eng
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Resource Type | departmental bulletin paper |
Publisher |
Hiroshima University School of Medicine
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Date of Issued | 1983-03 |
Publish Type | Version of Record |
Access Rights | open access |
Source Identifier |
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 6688069
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