Purification and Antigenic Specificity of Alpha Protein (Yoneda and Fukui) from Mycobacterium tuberculosis and Mycobacterium intracellulare

Mycobacterium tuberculosis および Mycobacterium intracellulare のαタンパク質（米田・福井）の精製法ならびに抗原特異性について

Tasaka Hiromichi

Kiyotani Katsuhiro

Matsuo Yoshiyasu

Hiroshima Journal of Medical Sciences

[PISSN] 0018-2052

[EISSN] 2433-7668

[NCID] AA00664312

Alpha protein antigen isolated from culture filtrate of Mycobacterium tuberculosis by Yoneda and Fukui (1961) was a cross-reacting material among mycobacteria. Purified alpha protein of M. tuberculosis obtained by a series of procedures with gel filtration, ion-exchange chromatographies, and chromatofocusing. Alpha protein of Mycobacterium intracellulare was purified by an affinity chromatography on anti M. tuberculosis-alpha serum conjugated sepharose. Alpha antigen of M. tuberculosis possessed specific antigenic determinants for both M. tuberculosis and M. bovis, and so did alpha antigen of M. intracellulare for both M. intracellulare and M. avium. Extracts from M. kansasii, M. scrofulaceum, M. gordonae, M. xenopi and M. gastri showed alpha antigenicity somewhat different from those of M. tuberculosis and M. intracellulare. Alpha antigen was not detected in extracts from M. nonchromogenicum, M. terrae and M. triviale.

Alpha protein

Mycobacterium tuberculosis

Mycobacterium intracellulare

Serology

医学 [ 490 ]

英語

Hiroshima University School of Medicine

[ISSN] 0018-2052

[NCID] AA00664312

[PMID] 6190783