Purification and Antigenic Specificity of Alpha Protein (Yoneda and Fukui) from Mycobacterium tuberculosis and Mycobacterium intracellulare

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Title ( eng )
Purification and Antigenic Specificity of Alpha Protein (Yoneda and Fukui) from Mycobacterium tuberculosis and Mycobacterium intracellulare
Title ( jpn )
Mycobacterium tuberculosis および Mycobacterium intracellulare のαタンパク質(米田・福井)の精製法ならびに抗原特異性について
Creator
Tasaka Hiromichi
Kiyotani Katsuhiro
Matsuo Yoshiyasu
Source Title
Hiroshima Journal of Medical Sciences
Volume 32
Issue 1
Start Page 1
End Page 8
Journal Identifire
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
Abstract
Alpha protein antigen isolated from culture filtrate of Mycobacterium tuberculosis by Yoneda and Fukui (1961) was a cross-reacting material among mycobacteria. Purified alpha protein of M. tuberculosis obtained by a series of procedures with gel filtration, ion-exchange chromatographies, and chromatofocusing. Alpha protein of Mycobacterium intracellulare was purified by an affinity chromatography on anti M. tuberculosis-alpha serum conjugated sepharose. Alpha antigen of M. tuberculosis possessed specific antigenic determinants for both M. tuberculosis and M. bovis, and so did alpha antigen of M. intracellulare for both M. intracellulare and M. avium. Extracts from M. kansasii, M. scrofulaceum, M. gordonae, M. xenopi and M. gastri showed alpha antigenicity somewhat different from those of M. tuberculosis and M. intracellulare. Alpha antigen was not detected in extracts from M. nonchromogenicum, M. terrae and M. triviale.
Keywords
Alpha protein
Mycobacterium tuberculosis
Mycobacterium intracellulare
Serology
NDC
Medical sciences [ 490 ]
Language
eng
Resource Type departmental bulletin paper
Publisher
Hiroshima University School of Medicine
Date of Issued 1983-03
Publish Type Version of Record
Access Rights open access
Source Identifier
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 6190783