Purification and Antigenic Specificity of Alpha Protein (Yoneda and Fukui) from Mycobacterium tuberculosis and Mycobacterium intracellulare
Hiroshima Journal of Medical Sciences Volume 32 Issue 1
Page 1-8
published_at 1983-03
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Title ( eng ) |
Purification and Antigenic Specificity of Alpha Protein (Yoneda and Fukui) from Mycobacterium tuberculosis and Mycobacterium intracellulare
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Title ( jpn ) |
Mycobacterium tuberculosis および Mycobacterium intracellulare のαタンパク質(米田・福井)の精製法ならびに抗原特異性について
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Creator |
Tasaka Hiromichi
Kiyotani Katsuhiro
Matsuo Yoshiyasu
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Source Title |
Hiroshima Journal of Medical Sciences
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Volume | 32 |
Issue | 1 |
Start Page | 1 |
End Page | 8 |
Journal Identifire |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
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Abstract |
Alpha protein antigen isolated from culture filtrate of Mycobacterium tuberculosis by Yoneda and Fukui (1961) was a cross-reacting material among mycobacteria. Purified alpha protein of M. tuberculosis obtained by a series of procedures with gel filtration, ion-exchange chromatographies, and chromatofocusing. Alpha protein of Mycobacterium intracellulare was purified by an affinity chromatography on anti M. tuberculosis-alpha serum conjugated sepharose. Alpha antigen of M. tuberculosis possessed specific antigenic determinants for both M. tuberculosis and M. bovis, and so did alpha antigen of M. intracellulare for both M. intracellulare and M. avium. Extracts from M. kansasii, M. scrofulaceum, M. gordonae, M. xenopi and M. gastri showed alpha antigenicity somewhat different from those of M. tuberculosis and M. intracellulare. Alpha antigen was not detected in extracts from M. nonchromogenicum, M. terrae and M. triviale.
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Keywords |
Alpha protein
Mycobacterium tuberculosis
Mycobacterium intracellulare
Serology
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NDC |
Medical sciences [ 490 ]
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Language |
eng
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Resource Type | departmental bulletin paper |
Publisher |
Hiroshima University School of Medicine
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Date of Issued | 1983-03 |
Publish Type | Version of Record |
Access Rights | open access |
Source Identifier |
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 6190783
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