Fluorometric Measurement of Neuraminidase Activity of Influenza Viruses
Hiroshima Journal of Medical Sciences 33 巻 2 号
287-292 頁
1984-06 発行
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| ファイル情報(添付) | |
| タイトル ( eng ) |
Fluorometric Measurement of Neuraminidase Activity of Influenza Viruses
|
| タイトル ( jpn ) |
螢光比色法によるインフルエンザウイルスノイラミニダーゼ活性の測定
|
| 作成者 |
Kiyotani Katsuhiro
Takei Naomi
Matsuo Yoshiyasu
|
| 収録物名 |
Hiroshima Journal of Medical Sciences
|
| 巻 | 33 |
| 号 | 2 |
| 開始ページ | 287 |
| 終了ページ | 292 |
| 収録物識別子 |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
|
| 抄録 |
A rapid and simple assay method for determining neuraminidase activity was investigated fluorometrically with 4-methylumbelliferyl-N-Ac-α-D-neuraminide as a substrate.
The time required for performance was less than 3 hr and the activity could be simply measured with a fluorescent spectrophotometer. Neuraminidase activity in culture supernatant of MDCK cell infected with a small quantity of influenza A/Aichi/2/68 virus was demonstrated by the fluorometric assay 24 hr after infection, when no viral cytopathogenic effect could be observed and no viral multiplication could be demonstrated by the hemagglutination test and the colorimetric neuraminidase assay method using fetuin as a substrate. The result was that the minimum number of virion detectable by the fluorometric neuraminidase assay method was approximately 10^3 order. |
| 著者キーワード |
4-Methylumbelliferyl-N-Ac-α-D-neuraminide
Neuraminidase
Influenza virus
|
| NDC分類 |
医学 [ 490 ]
|
| 言語 |
英語
|
| 資源タイプ | 紀要論文 |
| 出版者 |
Hiroshima University School of Medicine
|
| 発行日 | 1984-06 |
| 出版タイプ | Version of Record（出版社版。早期公開を含む） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 6480393
|
