Fluorometric Measurement of Neuraminidase Activity of Influenza Viruses
Hiroshima Journal of Medical Sciences Volume 33 Issue 2
Page 287-292
published_at 1984-06
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| File | |
| Title ( eng ) |
Fluorometric Measurement of Neuraminidase Activity of Influenza Viruses
|
| Title ( jpn ) |
螢光比色法によるインフルエンザウイルスノイラミニダーゼ活性の測定
|
| Creator |
Kiyotani Katsuhiro
Takei Naomi
Matsuo Yoshiyasu
|
| Source Title |
Hiroshima Journal of Medical Sciences
|
| Volume | 33 |
| Issue | 2 |
| Start Page | 287 |
| End Page | 292 |
| Journal Identifire |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
|
| Abstract |
A rapid and simple assay method for determining neuraminidase activity was investigated fluorometrically with 4-methylumbelliferyl-N-Ac-α-D-neuraminide as a substrate.
The time required for performance was less than 3 hr and the activity could be simply measured with a fluorescent spectrophotometer. Neuraminidase activity in culture supernatant of MDCK cell infected with a small quantity of influenza A/Aichi/2/68 virus was demonstrated by the fluorometric assay 24 hr after infection, when no viral cytopathogenic effect could be observed and no viral multiplication could be demonstrated by the hemagglutination test and the colorimetric neuraminidase assay method using fetuin as a substrate. The result was that the minimum number of virion detectable by the fluorometric neuraminidase assay method was approximately 10^3 order. |
| Keywords |
4-Methylumbelliferyl-N-Ac-α-D-neuraminide
Neuraminidase
Influenza virus
|
| NDC |
Medical sciences [ 490 ]
|
| Language |
eng
|
| Resource Type | departmental bulletin paper |
| Publisher |
Hiroshima University School of Medicine
|
| Date of Issued | 1984-06 |
| Publish Type | Version of Record |
| Access Rights | open access |
| Source Identifier |
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 6480393
|
