The Effect of Phorbol Esters on Cell Growth and Epidermal Growth Factor Receptor Modulation in a Human Gastric Carcinoma Cell Line TMK-1
Hiroshima Journal of Medical Sciences 38 巻 4 号
191-195 頁
1989-12 発行
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| ファイル情報(添付) | |
| タイトル ( eng ) |
The Effect of Phorbol Esters on Cell Growth and Epidermal Growth Factor Receptor Modulation in a Human Gastric Carcinoma Cell Line TMK-1
|
| 作成者 |
Ochiai Atsushi
Kameda Takashi
Takanashi Atsushi
Takekura Naoki
Tahara Eiichi
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| 収録物名 |
Hiroshima Journal of Medical Sciences
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| 巻 | 38 |
| 号 | 4 |
| 開始ページ | 191 |
| 終了ページ | 195 |
| 収録物識別子 |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
|
| 抄録 |
Tumor promoting phorbol esters, 12-O-tetradecanoylphorbol-13-acetate (TPA) and phorbol-12, 13-dibutyrate (PDBu), significantly enhanced the growth of human gastric cancer cell line TMK-1, whilst activating protein kinase C. The time course of 125I-epidermal growth factor (EGF) binding to TMK-1 cells after TPA treatment showed a decrease in the number of EGF receptors on TMK-1 cells within 3 hr. Autophosphorylation of EGF receptor decreased in accordance with the decrease of EGF binding by TPA treatment. Scatchard plot analysis of TMK-1 cells after TPA treatment showed that high affinity EGF receptor disappeared at 3hr but the number of EGF receptors increased at 24 hr. These findings suggest that tumor promoting phorbol esters stimulate the cell growth through activation of protein kinase C and modification of EGF receptor of human gastric cancer cell line TMK-1.
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| 著者キーワード |
Phorbol ester
Protein kinase C
Gastric cancer cell
EGF receptor
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| 内容記述 |
This research was supported in part by Grants-in Aid for Cancer Research from the Ministry of Education, Science and Culture of Japan and from the Ministry of Health and Welfare for a Comprehensive 10-Year Strategy for Cancer Control, Japan.
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| NDC分類 |
医学 [ 490 ]
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| 言語 |
英語
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| 資源タイプ | 紀要論文 |
| 出版者 |
Hiroshima University Medical Press
|
| 発行日 | 1989-12 |
| 出版タイプ | Version of Record（出版社版。早期公開を含む） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 2637248
|
