The Effect of Phorbol Esters on Cell Growth and Epidermal Growth Factor Receptor Modulation in a Human Gastric Carcinoma Cell Line TMK-1
Hiroshima Journal of Medical Sciences Volume 38 Issue 4
Page 191-195
published_at 1989-12
アクセス数 : 1027 件
ダウンロード数 : 99 件
今月のアクセス数 : 1 件
今月のダウンロード数 : 1 件
この文献の参照には次のURLをご利用ください : https://ir.lib.hiroshima-u.ac.jp/00038130
| File | |
| Title ( eng ) |
The Effect of Phorbol Esters on Cell Growth and Epidermal Growth Factor Receptor Modulation in a Human Gastric Carcinoma Cell Line TMK-1
|
| Creator |
Ochiai Atsushi
Kameda Takashi
Takanashi Atsushi
Takekura Naoki
Tahara Eiichi
|
| Source Title |
Hiroshima Journal of Medical Sciences
|
| Volume | 38 |
| Issue | 4 |
| Start Page | 191 |
| End Page | 195 |
| Journal Identifire |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
|
| Abstract |
Tumor promoting phorbol esters, 12-O-tetradecanoylphorbol-13-acetate (TPA) and phorbol-12, 13-dibutyrate (PDBu), significantly enhanced the growth of human gastric cancer cell line TMK-1, whilst activating protein kinase C. The time course of 125I-epidermal growth factor (EGF) binding to TMK-1 cells after TPA treatment showed a decrease in the number of EGF receptors on TMK-1 cells within 3 hr. Autophosphorylation of EGF receptor decreased in accordance with the decrease of EGF binding by TPA treatment. Scatchard plot analysis of TMK-1 cells after TPA treatment showed that high affinity EGF receptor disappeared at 3hr but the number of EGF receptors increased at 24 hr. These findings suggest that tumor promoting phorbol esters stimulate the cell growth through activation of protein kinase C and modification of EGF receptor of human gastric cancer cell line TMK-1.
|
| Keywords |
Phorbol ester
Protein kinase C
Gastric cancer cell
EGF receptor
|
| Descriptions |
This research was supported in part by Grants-in Aid for Cancer Research from the Ministry of Education, Science and Culture of Japan and from the Ministry of Health and Welfare for a Comprehensive 10-Year Strategy for Cancer Control, Japan.
|
| NDC |
Medical sciences [ 490 ]
|
| Language |
eng
|
| Resource Type | departmental bulletin paper |
| Publisher |
Hiroshima University Medical Press
|
| Date of Issued | 1989-12 |
| Publish Type | Version of Record |
| Access Rights | open access |
| Source Identifier |
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 2637248
|
