Automated Microanalysis of Creatinine by Coupled Enzyme Reactions
Hiroshima Journal of Medical Sciences 41 巻 1 号
1-5 頁
1992-03 発行
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| ファイル情報(添付) | |
| タイトル ( eng ) |
Automated Microanalysis of Creatinine by Coupled Enzyme Reactions
|
| 作成者 |
Asano Kenji
Iwasaki Hiroya
Fujimura Katsuyuki
Ikeda Mitsunori
Sugimoto Yoshimi
Matsubara Akemi
Yano Kazuko
Irisawa Hiroko
Kono Fujiko
Kanbe Masayuki
Tsubokura Tokuo
Inouye Yoshio
Nakamura Shoshiro
|
| 収録物名 |
Hiroshima Journal of Medical Sciences
|
| 巻 | 41 |
| 号 | 1 |
| 開始ページ | 1 |
| 終了ページ | 5 |
| 収録物識別子 |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
|
| 抄録 |
Hydrogen peroxide was generated from creatinine by the sequential enzyme reactions of creatinine amidohydrolase, creatine amidinohydrolase and sarcosine oxidase. Hydrogen peroxide was, in turn, used stoichiometrically for the condensation of 4-aminoantipyrine and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-m-toluidine catalyzed by horse-radish peroxidase, resulting in the formation of quinone dye with maximum absorbance at 546 nm. The optimized assay conditions for the enzymatic determination of creatinine in a HITACHI 7250 autoanalyzer was established. This system, which requires less than 5 μl of sample, was found to be the most economical for laboratories equipped with autoanalyzers.
|
| 著者キーワード |
Creatinine
Creatinine amidohydrolase
Creatine amidinohydrolase
Sarcosine oxidase
|
| NDC分類 |
医学 [ 490 ]
|
| 言語 |
英語
|
| 資源タイプ | 紀要論文 |
| 出版者 |
Hiroshima University Medical Press
|
| 発行日 | 1992-03 |
| 出版タイプ | Version of Record（出版社版。早期公開を含む） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 1572805
|
