Automated Microanalysis of Creatinine by Coupled Enzyme Reactions
Hiroshima Journal of Medical Sciences Volume 41 Issue 1
Page 1-5
published_at 1992-03
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| File | |
| Title ( eng ) |
Automated Microanalysis of Creatinine by Coupled Enzyme Reactions
|
| Creator |
Asano Kenji
Iwasaki Hiroya
Fujimura Katsuyuki
Ikeda Mitsunori
Sugimoto Yoshimi
Matsubara Akemi
Yano Kazuko
Irisawa Hiroko
Kono Fujiko
Kanbe Masayuki
Tsubokura Tokuo
Inouye Yoshio
Nakamura Shoshiro
|
| Source Title |
Hiroshima Journal of Medical Sciences
|
| Volume | 41 |
| Issue | 1 |
| Start Page | 1 |
| End Page | 5 |
| Journal Identifire |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
|
| Abstract |
Hydrogen peroxide was generated from creatinine by the sequential enzyme reactions of creatinine amidohydrolase, creatine amidinohydrolase and sarcosine oxidase. Hydrogen peroxide was, in turn, used stoichiometrically for the condensation of 4-aminoantipyrine and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-m-toluidine catalyzed by horse-radish peroxidase, resulting in the formation of quinone dye with maximum absorbance at 546 nm. The optimized assay conditions for the enzymatic determination of creatinine in a HITACHI 7250 autoanalyzer was established. This system, which requires less than 5 μl of sample, was found to be the most economical for laboratories equipped with autoanalyzers.
|
| Keywords |
Creatinine
Creatinine amidohydrolase
Creatine amidinohydrolase
Sarcosine oxidase
|
| NDC |
Medical sciences [ 490 ]
|
| Language |
eng
|
| Resource Type | departmental bulletin paper |
| Publisher |
Hiroshima University Medical Press
|
| Date of Issued | 1992-03 |
| Publish Type | Version of Record |
| Access Rights | open access |
| Source Identifier |
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 1572805
|
