Homozygosity Mapping on Homozygosity Haplotype Analysis to Detect Recessive Disease-Causing Genes from a Small Number of Unrelated, Outbred Patients

PLoS ONE Volume 6 Issue 9 Page e25059- published_at 2011
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Title ( eng )
Homozygosity Mapping on Homozygosity Haplotype Analysis to Detect Recessive Disease-Causing Genes from a Small Number of Unrelated, Outbred Patients
Creator
Hagiwara Koichi
Shiihara Jun
Tanaka Tomoaki
Miyazawa Hitoshi
Suzuki Tomoko
Kohda Masakazu
Okazaki Yasushi
Seyama Kuniaki
Source Title
PLoS ONE
Volume 6
Issue 9
Start Page e25059
Abstract
Genes involved in disease that are not common are often difficult to identify; a method that pinpoints them from a small number of unrelated patients will be of great help. In order to establish such a method that detects recessive genes identical-by-descent, we modified homozygosity mapping (HM) so that it is constructed on the basis of homozygosity haplotype (HM on HH) analysis. An analysis using 6 unrelated patients with Siiyama-type alpha 1-antitrypsin deficiency, a disease caused by a founder gene, the correct gene locus was pinpointed from data of any 2 patients (length: 1.2-21.8 centimorgans, median: 1.6 centimorgans). For a test population in which these 6 patients and 54 healthy subjects were scrambled, the approach accurately identified these 6 patients and pinpointed the locus to a 1.4-centimorgan fragment. Analyses using synthetic data revealed that the analysis works well for IBD fragment derived from a most recent common ancestor (MRCA) who existed less than 60 generations ago. The analysis is unsuitable for the genes with a frequency in general population more than 0.1. Thus, HM on HH analysis is a powerful technique, applicable to a small number of patients not known to be related, and will accelerate the identification of disease-causing genes for recessive conditions.
NDC
Medical sciences [ 490 ]
Language
eng
Resource Type journal article
Publisher
Public Library of Science
Date of Issued 2011
Rights
(c) 2011 Hagiwara et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Publish Type Version of Record
Access Rights open access
Source Identifier
[ISSN] 1932-6203
[DOI] 10.1371/journal.pone.0025059
[DOI] http://dx.doi.org/10.1371/journal.pone.0025059