Shed Blood-derived Cells from Total Hip Arthroplasty Have Osteoinductive Potential : A Pilot Study

Yoshida Tomokazu

Ishikawa Masakazu

Yasunaga Yuji

Yamasaki Takuma

Ochi Mitsuo

Clinical Orthopaedics and Related Research

Background: Cell therapy using autologous cells has been used in the treatment of various medical conditions. The mononuclear cell (MNC) fraction of bone marrow (BM) contains stem/progenitor cells that could contribute to osteogenesis and angiogenesis.

Questions/purposes: We asked whether MNCs derived from intraoperative shed blood (SB), consisting of peripheral blood and BM, have osteoinductive and angiogenic potential.

Methods: We harvested SB and BM from six patients undergoing THA. Isolated MNCs from SB and BM were analyzed by flow cytometry to evaluate the CD34+ cell fraction and 1 × 106 cells were seeded on an interconnective porous calcium hydroxyapatite ceramic (IP-CHA) and transplanted in the backs of athymic rats. IP-CHAs without cells were transplanted as controls and all composites were harvested after 4 and 8 weeks. Osteoinductive potential was evaluated by histologic observation, immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA) using anti-osteocalcin (OC) antibodies qualitatively and quantitatively. To evaluate angiogenic potential, capillary density was measured by immunohistochemistry using Isolectin B4 4 weeks after implantation.

Results: We found that CD34+ cells existed in SB-MNCs and there was a trend toward lower frequency compared with BM-MNCs. Histologic osteoinduction, OC expression, and capillary density were increased by transplantation of MNCs from SB. Similar results were achieved with MNCs from BM.

Conclusions: MNCs from SB have equivalent osteoinductive and angiogenic potential compared with those from BM.

Clinical Relevance: SB could be an attractive source for isolation of MNCs, enhancing osteoinduction and neovascularization, to augment the reconstruction of skeletal defects.

医学 [ 490 ]

英語

springer

Copyright (c) 2010 Springer

The original publication is available at www.springerlink.com

[ISSN] 0009-921X

[DOI] 10.1007/s11999-010-1444-z

[NCID] AA00607942

[DOI] http://dx.doi.org/10.1007/s11999-010-1444-z