Shed Blood-derived Cells from Total Hip Arthroplasty Have Osteoinductive Potential : A Pilot Study
Clinical Orthopaedics and Related Research Volume 468 Issue 10
Page 2725-2733
published_at 2010-10
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| File | |
| Title ( eng ) |
Shed Blood-derived Cells from Total Hip Arthroplasty Have Osteoinductive Potential : A Pilot Study
|
| Creator |
Yoshida Tomokazu
|
| Source Title |
Clinical Orthopaedics and Related Research
|
| Volume | 468 |
| Issue | 10 |
| Start Page | 2725 |
| End Page | 2733 |
| Abstract |
Background: Cell therapy using autologous cells has been used in the treatment of various medical conditions. The mononuclear cell (MNC) fraction of bone marrow (BM) contains stem/progenitor cells that could contribute to osteogenesis and angiogenesis.
Questions/purposes: We asked whether MNCs derived from intraoperative shed blood (SB), consisting of peripheral blood and BM, have osteoinductive and angiogenic potential. Methods: We harvested SB and BM from six patients undergoing THA. Isolated MNCs from SB and BM were analyzed by flow cytometry to evaluate the CD34+ cell fraction and 1 × 106 cells were seeded on an interconnective porous calcium hydroxyapatite ceramic (IP-CHA) and transplanted in the backs of athymic rats. IP-CHAs without cells were transplanted as controls and all composites were harvested after 4 and 8 weeks. Osteoinductive potential was evaluated by histologic observation, immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA) using anti-osteocalcin (OC) antibodies qualitatively and quantitatively. To evaluate angiogenic potential, capillary density was measured by immunohistochemistry using Isolectin B4 4 weeks after implantation. Results: We found that CD34+ cells existed in SB-MNCs and there was a trend toward lower frequency compared with BM-MNCs. Histologic osteoinduction, OC expression, and capillary density were increased by transplantation of MNCs from SB. Similar results were achieved with MNCs from BM. Conclusions: MNCs from SB have equivalent osteoinductive and angiogenic potential compared with those from BM. Clinical Relevance: SB could be an attractive source for isolation of MNCs, enhancing osteoinduction and neovascularization, to augment the reconstruction of skeletal defects. |
| NDC |
Medical sciences [ 490 ]
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| Language |
eng
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| Resource Type | journal article |
| Publisher |
springer
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| Date of Issued | 2010-10 |
| Rights |
Copyright (c) 2010 Springer
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| Publish Type | Author’s Original |
| Access Rights | open access |
| Source Identifier |
The original publication is available at www.springerlink.com
[ISSN] 0009-921X
[DOI] 10.1007/s11999-010-1444-z
[NCID] AA00607942
[DOI] http://dx.doi.org/10.1007/s11999-010-1444-z
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