Absence of viral interference and different susceptibility to interferon between hepatitis B virus and hepatitis C virus in human hepatocyte chimeric mice

Journal of Hepatology Volume 51 Issue 6 Page 1046-1054 published_at 2009-12
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Title ( eng )
Absence of viral interference and different susceptibility to interferon between hepatitis B virus and hepatitis C virus in human hepatocyte chimeric mice
Creator
Hatakeyama Tsuyoshi
Kitamura Shosuke
Mitsui Fukiko
Takahashi Shoichi
Maekawa Toshiro
Tateno Chise
Wakita Takaji
Source Title
Journal of Hepatology
Volume 51
Issue 6
Start Page 1046
End Page 1054
Abstract
Background/Aims: Both hepatitis B virus (HBV) and hepatitis C virus (HCV) replicate in the liver and show resistance against innate immunity and interferon (IFN) treatment. Whether there is interference between these two viruses is still controversial. We investigated the interference between these two viruses and the mode of resistance against IFN. Methods: We performed infection experiments with either or both of the two hepatitis viruses in human hepatocyte chimeric mice. Huh7 cell lines with stable production of HBV were also established and transfected with HCV JFH1 clone. Mice and cell lines were treated with IFN. The viral levels in mice sera and culture supernatants and messenger RNA levels of IFN-stimulated genes were measured. Results: No apparent interference between the two viruses was seen ill vivo. Only a small (0.3 log) reduction in serum HBV and a rapid reduction in HCV were observed after IFN treatment, regardless of infection with the other virus. In ill vitro studies, no interference between the two viruses was observed. The effect of IFN on each virus was not affected by the presence of the other virus. IFN-induced reductions of viruses in culture supernatants were similar to those in ill vivo study. Conclusions: No interference between the two hepatitis viruses exists in the liver in the absence of hepatitis. The mechanisms of IFN resistance of the two viruses target different areas of the IFN system.
Keywords
Superinfection
JFH-1
IFN-stimulated genes
NDC
Medical sciences [ 490 ]
Language
eng
Resource Type journal article
Publisher
Elsevier Science Bv
Date of Issued 2009-12
Rights
Copyright (c) 2009 European Association for the Study of the Liver. Published by Elsevier B.V.
Publish Type Author’s Original
Access Rights open access
Source Identifier
[ISSN] 0168-8278
[DOI] 10.1016/j.jhep.2009.09.002
[NCID] AA10459105
[DOI] http://dx.doi.org/10.1016/j.jhep.2009.09.002