P34^<cdc2> Kinase and MAP Kinase Activities and Parthenogenetic Activation in Porcine Oocytes after Injection of Miniature Pig Sperm Extracts
Journal of mammalian ova research = 日本哺乳動物卵子学会誌 25 巻 1 号
63-68 頁
2008-04-01 発行
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| ファイル情報(添付) | |
| タイトル ( eng ) |
P34^<cdc2> Kinase and MAP Kinase Activities and Parthenogenetic Activation in Porcine Oocytes after Injection of Miniature Pig Sperm Extracts
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| タイトル ( jpn ) |
ミニブタ精子抽出物を注入したブタ卵子における p34^<cdc2> kinase と MAP kinase 活性および人為的活性化
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| 作成者 |
Matsuura Daizou
|
| 収録物名 |
Journal of mammalian ova research = 日本哺乳動物卵子学会誌
|
| 巻 | 25 |
| 号 | 1 |
| 開始ページ | 63 |
| 終了ページ | 68 |
| 抄録 |
The aim of the present study was to examine the rate of activation and time-dependent changes in p34cdc2 kinase and MAP kinase activities in porcine oocytes after injection of sperm extracts (SE) or treatment with Ca2+ ionophore to clarify whether SE injection is useful for porcine oocyte activation. SE was prepared from miniature pig sperm by non-ionic surfactant. Oocytes that were treated with Ca2+ ionophore and injected with SE were activated at rates of 41% and 46%, respectively. The activities of p34cdc2 kinase and MAP kinase in each culture period (4, 8 and 12 h) in the Ca2+ ionophore group and SE injection group were significantly lower than those in the control group. These results suggest that SE injection induces decrease of p34cdc2 kinase and MAP kinase activity, and induces oocyte activation, as well as Ca2+ ionophore treatment, but the induction was comparatively limited.
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| 著者キーワード |
Oocyte activation
MAP kinase
P34^<cdc2> kinase
Sperm extracts
Miniature pig
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| NDC分類 |
動物学 [ 480 ]
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| 言語 |
英語
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| 資源タイプ | 学術雑誌論文 |
| 出版者 |
日本哺乳動物卵子学会
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| 発行日 | 2008-04-01 |
| 出版タイプ | Version of Record（出版社版。早期公開を含む） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
[ISSN] 1341-7738
[DOI] 10.1274/jmor.25.63
[NCID] AN10548943
[DOI] http://dx.doi.org/10.1274/jmor.25.63
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