P34^<cdc2> Kinase and MAP Kinase Activities and Parthenogenetic Activation in Porcine Oocytes after Injection of Miniature Pig Sperm Extracts

ミニブタ精子抽出物を注入したブタ卵子における p34^<cdc2> kinase と MAP kinase 活性および人為的活性化

Matsuura Daizou

Maeda Teruo

Journal of mammalian ova research = 日本哺乳動物卵子学会誌

The aim of the present study was to examine the rate of activation and time-dependent changes in p34cdc2 kinase and MAP kinase activities in porcine oocytes after injection of sperm extracts (SE) or treatment with Ca2+ ionophore to clarify whether SE injection is useful for porcine oocyte activation. SE was prepared from miniature pig sperm by non-ionic surfactant. Oocytes that were treated with Ca2+ ionophore and injected with SE were activated at rates of 41% and 46%, respectively. The activities of p34cdc2 kinase and MAP kinase in each culture period (4, 8 and 12 h) in the Ca2+ ionophore group and SE injection group were significantly lower than those in the control group. These results suggest that SE injection induces decrease of p34cdc2 kinase and MAP kinase activity, and induces oocyte activation, as well as Ca2+ ionophore treatment, but the induction was comparatively limited.

Oocyte activation

MAP kinase

P34^<cdc2> kinase

Sperm extracts

Miniature pig

Zoology [ 480 ]

eng

日本哺乳動物卵子学会

[ISSN] 1341-7738

[DOI] 10.1274/jmor.25.63

[NCID] AN10548943

[DOI] http://dx.doi.org/10.1274/jmor.25.63