Mizoribine Suppresses the Progression of Experimental Peritoneal Fibrosis in a Rat Model
Nephron Experimental Nephrology Volume 112 Issue 2
Page E59-E69
published_at 2009
アクセス数 : 1228 件
ダウンロード数 : 287 件
今月のアクセス数 : 3 件
今月のダウンロード数 : 1 件
この文献の参照には次のURLをご利用ください : https://ir.lib.hiroshima-u.ac.jp/00027694
File | |
Title ( eng ) |
Mizoribine Suppresses the Progression of Experimental Peritoneal Fibrosis in a Rat Model
|
Creator |
Takahashi Shunsuke
Taniguchi Yoshihiko
Arakawa Tetsuji
Kawai Toru
Ito Takafumi
Yorioka Noriaki
|
Source Title |
Nephron Experimental Nephrology
|
Volume | 112 |
Issue | 2 |
Start Page | E59 |
End Page | E69 |
Abstract |
Background/Aims: Peritoneal fibrosis is a serious complication of peritoneal dialysis (PD). It has been reported that administration of mizoribine, an effective immunosuppressant, ameliorated renal fibrosis in a rat model of unilateral ureteral obstruction. We therefore examined the effects of mizoribine in an experimental model of peritoneal fibrosis. Methods: 24 rats were given a daily intraperitoneal injection of chlorhexidine gluconate and ethanol dissolved in saline. The rats were divided into three groups (n = 8 per group) that received either vehicle or mizoribine at a dose of 2 or 8 mg/kg once a day. 28 days after the start of the treatments the rats were sacrificed and peritoneal tissue samples collected. Macrophage infiltration (ED1), myofibroblast accumulation (alpha-smooth muscle actin (SMA)) and expression of type III collagen, transforming growth factor (TGF)-beta and monocyte chemotactic protein-1 (MCP-1) were examined by immunohistochemistry. Results: Mizoribine significantly suppressed submesothelial zone thickening and reduced macrophage infiltration. Mizoribine also reduced collagen III+ area and decreased the number of alpha-SMA(+), TGF-beta(+) and MCP-1(+) cells. The magnitude of the changes observed was dose-dependent. Conclusion: The administration of mizoribine prevented the progression of peritoneal fibrosis in this rat model. Mizoribine may represent a novel therapy for peritoneal sclerosis in patients undergoing long-term PD.
|
Keywords |
Macrophage
Mizoribine
Peritoneal dialysis
Peritoneal fibrosis
Experimental peritoneal fibrosis
rat model
|
NDC |
Medical sciences [ 490 ]
|
Language |
eng
|
Resource Type | journal article |
Publisher |
Karger
|
Date of Issued | 2009 |
Rights |
Copyright (c) 2009 S. Karger AG, Basel
|
Publish Type | Author’s Original |
Access Rights | open access |
Source Identifier |
[ISSN] 1660-2129
[DOI] 10.1159/000213896
[DOI] http://dx.doi.org/10.1159/000213896
|