Sensitive determination of anions in saliva using capillary electrophoresis after transient isotachophoretic preconcentration

Talanta 77 巻 1 号 278-281 頁 2008-10-19 発行
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タイトル ( eng )
Sensitive determination of anions in saliva using capillary electrophoresis after transient isotachophoretic preconcentration
作成者
Xu Zhongqi
Doi Takayuki
Timerbaev Andrei R.
収録物名
Talanta
77
1
開始ページ 278
終了ページ 281
抄録
A transient isotachophoresis-capillary electrophoresis (tITP-CE) system for the determination of minor inorganic anions in saliva is described. The complete separation and quantification of bromide, iodide, nitrate, nitrite, and thiocyanate has been achieved with only centrifugation and dilution of the saliva sample. In-line tITP preconcentration conditions, created by introduction of the plugs of 5 mM dithionic acid (leading electrolyte) and 10 mM formic acid (terminating electrolyte) before and after the sample zone, respectively, allowed the limits of direct UV absorption detection (at 200 nm) to be up to 50-fold improved as compared with CE without tITP. As a result, nitrate and thiocyanate were still detectable at 4.6 and 3.8 µg l-1, respectively, in 1000 times diluted saliva. The daily variations of anionic concentrations in saliva samples taken from a smoking health volunteer were discussed based on the results of tITP-CE analysis. It was confirmed that the thiocyanate concentration in saliva noticeably increased after smoking. This is apparently the first report on simultaneous quantification of more than four anionic salivary constituents using CE.
著者キーワード
Inorganic anions
Capillary zone electrophoresis
Preconcentration
Transient isotachophoresis
Saliva
NDC分類
生物科学・一般生物学 [ 460 ]
言語
英語
資源タイプ 学術雑誌論文
出版者
Elsevier Science BV
発行日 2008-10-19
権利情報
Copyright (c) 2008 Elsevier B.V.
出版タイプ Author’s Original(十分な品質であるとして、著者から正式な査読に提出される版)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 0039-9140
[DOI] 10.1016/j.talanta.2008.06.017
[NCID] AA00856594
[DOI] http://dx.doi.org/10.1016/j.talanta.2008.06.017