Sensitive determination of anions in saliva using capillary electrophoresis after transient isotachophoretic preconcentration

Talanta Volume 77 Issue 1 Page 278-281 published_at 2008-10-19
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Title ( eng )
Sensitive determination of anions in saliva using capillary electrophoresis after transient isotachophoretic preconcentration
Creator
Xu Zhongqi
Doi Takayuki
Timerbaev Andrei R.
Source Title
Talanta
Volume 77
Issue 1
Start Page 278
End Page 281
Abstract
A transient isotachophoresis-capillary electrophoresis (tITP-CE) system for the determination of minor inorganic anions in saliva is described. The complete separation and quantification of bromide, iodide, nitrate, nitrite, and thiocyanate has been achieved with only centrifugation and dilution of the saliva sample. In-line tITP preconcentration conditions, created by introduction of the plugs of 5 mM dithionic acid (leading electrolyte) and 10 mM formic acid (terminating electrolyte) before and after the sample zone, respectively, allowed the limits of direct UV absorption detection (at 200 nm) to be up to 50-fold improved as compared with CE without tITP. As a result, nitrate and thiocyanate were still detectable at 4.6 and 3.8 µg l-1, respectively, in 1000 times diluted saliva. The daily variations of anionic concentrations in saliva samples taken from a smoking health volunteer were discussed based on the results of tITP-CE analysis. It was confirmed that the thiocyanate concentration in saliva noticeably increased after smoking. This is apparently the first report on simultaneous quantification of more than four anionic salivary constituents using CE.
Keywords
Inorganic anions
Capillary zone electrophoresis
Preconcentration
Transient isotachophoresis
Saliva
NDC
Biology [ 460 ]
Language
eng
Resource Type journal article
Publisher
Elsevier Science BV
Date of Issued 2008-10-19
Rights
Copyright (c) 2008 Elsevier B.V.
Publish Type Author’s Original
Access Rights open access
Source Identifier
[ISSN] 0039-9140
[DOI] 10.1016/j.talanta.2008.06.017
[NCID] AA00856594
[DOI] http://dx.doi.org/10.1016/j.talanta.2008.06.017