Exploration of the genes responsible for unlimited proliferation of immortalized lung fibroblasts

Hiyama Keiko

Tanimoto Keiji

Nishimura Youhei

Tsugane Momomi

Fukuba Ikuko

Sotomaru Yusuke

Hiyama Eiso

Nishiyama Masahiko

Experimental Lung Research

Regulation mechanism of lung fibroblast proliferation remains unknown. To elucidate the key molecules in it, we here established mortal and immortal non-transformed lung fibroblast cell line/strains with elongated lifespan by telomerase reversetranscriptase gene transfection. Comparing the expression profiles of them, 51 genes were explored to be the candidates responsible for regulation of cellular proliferation of lung fibroblasts. This set of fibrobrast strains of same origin with different proliferative capacities may become useful model cells for research on lung fibroblast growth regulation and the candidate genes explored in this study may provide biomarkers or therapeutic targets of pulmonary fibrosis.

lung fibroblast

microarray

growth regulation

proliferation

telomerase

Medical sciences [ 490 ]

eng

Taylor & Francis Inc

Copyright (c) 2008 Taylor & Francis

This is a preprint of an article submitted for consideration in the Experimental Lung Research © 2008 copyright Taylor & Francis; Experimental Lung Research is available online at informaworldTM with the open URL of your article;

[ISSN] 0190-2148

[DOI] 10.1080/01902140802221912

[NCID] AA00181680

[DOI] http://dx.doi.org/10.1080/01902140802221912