Suppression of inducible nitric oxide synthase and cyclooxygenase-2 gene expression by 22(R)-hydroxycholesterol requires de novo protein synthesis in activated macrophages
The Journal of Steroid Biochemistry and Molecular Biology 97 巻 4 号
376-383 頁
2005-12 発行
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| ファイル情報(添付) | |
| タイトル ( eng ) |
Suppression of inducible nitric oxide synthase and cyclooxygenase-2 gene expression by 22(R)-hydroxycholesterol requires de novo protein synthesis in activated macrophages
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| 作成者 |
Yuge Osafumi
Ninomiya Yuichi
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| 収録物名 |
The Journal of Steroid Biochemistry and Molecular Biology
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| 巻 | 97 |
| 号 | 4 |
| 開始ページ | 376 |
| 終了ページ | 383 |
| 抄録 |
Liver X receptors (LXRs) play an important role in lipid metabolism. Recently, a role for these proteins was identified in suppressing the inflammatory response. However, it is not known whether the natural ligands of LXRs, e.g. 22(R)-hydroxycholesterol (22R-HC), can suppress the inflammatory response after the onset of inflammation. We demonstrate here that treatment of Lipopolysaccharide (LPS)-activated RAW264.7 macrophages with 22R-HC markedly suppressed nitric oxide (NO) production and inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) mRNA expression. Additionally, 22R-HC did not affect the DNA binding activity of NF-κB, AP-1 and C/EBP(s), important transcriptional factors for iNOS and COX-2 genes expression. Furthermore iNOS and COX-2 mRNA suppression by 22R-HC was diminished by cellular treatment with cycloheximide. These results suggest that 22R-HC suppresses the expression of iNOS and COX-2 genes through de novo protein synthesis of an unidentified protein in LPS-activated macrophages. © 2005 Elsevier Ltd. All rights reserved.
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| 著者キーワード |
22(R)-Hydroxycholesterol
Inflammation
Macrophage
22R-HC
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| NDC分類 |
医学 [ 490 ]
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| 言語 |
英語
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| 資源タイプ | 学術雑誌論文 |
| 出版者 |
Elsevier Ltd
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| 発行日 | 2005-12 |
| 権利情報 |
Copyright (c) 2005 Elsevier Ltd
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| 出版タイプ | Author’s Original（十分な品質であるとして、著者から正式な査読に提出される版） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
[ISSN] 0960-0760
[DOI] 10.1016/j.jsbmb.2005.06.030
[PMID] 16146692
[DOI] http://dx.doi.org/10.1016/j.jsbmb.2005.06.030
～の異版である
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