Chloride Channel in Vanadocytes of a Vanadium-Rich Ascidian Ascidia sydneiensis samea

Comparative biochemistry and physiology. B, Biochemistry 136 巻 91-98 頁 2003 発行
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ファイル情報(添付)
タイトル ( eng )
Chloride Channel in Vanadocytes of a Vanadium-Rich Ascidian Ascidia sydneiensis samea
作成者
Yamaguchi Nobuo
Michibata Hitoshi
収録物名
Comparative biochemistry and physiology. B, Biochemistry
136
開始ページ 91
終了ページ 98
抄録
Ascidians, so-called sea squirts, can accumulate high levels of vanadium in the vacuoles of signet ring cells, which are one type of ascidian blood cell and are also called vanadocytes. In addition to containing high concentrations of vanadium in the +3 oxidation state, the proton concentrations in vanadocyte vacuoles are extremely high. In order to elucidate the entire mechanism of the accumulation and reduction of vanadium by ascidian vanadocytes, it is necessary to clarify the participation of anions, which might be involved as counter ions in the active accumulation of both vanadium and protons. We examined the chloride channel, since chloride ions are necessary for the acidification of intracellular vesicles and coexist with H+ ATPase. We cloned cDNA encoding a chloride channel from blood cells of a vanadium-rich ascidian, Ascidia sydneiensis samea. It encoded a 787-amino-acid protein, which showed striking similarity to mammalian ClC3/4/5-type chloride channels. Using a whole-mount in situ hybridization method that we developed for ascidian blood cells, the chloride channel was revealed to be transcribed in vanadocytes, suggesting its participation in the process of vanadium accumulation.
著者キーワード
Ascidian
Blood Cells
Chloride Channel
CLC-type
Gene Expression
H+-ATPase
Metal Accumulation
Vanadium
NDC分類
化学 [ 430 ]
言語
英語
資源タイプ 学術雑誌論文
出版者
Elsevier Science Inc
発行日 2003
権利情報
Copyright (c) 2003 Elsevier Science Inc
出版タイプ Author’s Original(十分な品質であるとして、著者から正式な査読に提出される版)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 1096-4959
[DOI] 10.1016/S1096-4959(03)00175-1
[DOI] http://dx.doi.org/10.1016/S1096-4959(03)00175-1 ~の異版である