Chloride Channel in Vanadocytes of a Vanadium-Rich Ascidian Ascidia sydneiensis samea

Comparative biochemistry and physiology. B, Biochemistry Volume 136 Page 91-98 published_at 2003
アクセス数 : 1602
ダウンロード数 : 280

今月のアクセス数 : 8
今月のダウンロード数 : 3
File
Title ( eng )
Chloride Channel in Vanadocytes of a Vanadium-Rich Ascidian Ascidia sydneiensis samea
Creator
Yamaguchi Nobuo
Michibata Hitoshi
Source Title
Comparative biochemistry and physiology. B, Biochemistry
Volume 136
Start Page 91
End Page 98
Abstract
Ascidians, so-called sea squirts, can accumulate high levels of vanadium in the vacuoles of signet ring cells, which are one type of ascidian blood cell and are also called vanadocytes. In addition to containing high concentrations of vanadium in the +3 oxidation state, the proton concentrations in vanadocyte vacuoles are extremely high. In order to elucidate the entire mechanism of the accumulation and reduction of vanadium by ascidian vanadocytes, it is necessary to clarify the participation of anions, which might be involved as counter ions in the active accumulation of both vanadium and protons. We examined the chloride channel, since chloride ions are necessary for the acidification of intracellular vesicles and coexist with H+ ATPase. We cloned cDNA encoding a chloride channel from blood cells of a vanadium-rich ascidian, Ascidia sydneiensis samea. It encoded a 787-amino-acid protein, which showed striking similarity to mammalian ClC3/4/5-type chloride channels. Using a whole-mount in situ hybridization method that we developed for ascidian blood cells, the chloride channel was revealed to be transcribed in vanadocytes, suggesting its participation in the process of vanadium accumulation.
Keywords
Ascidian
Blood Cells
Chloride Channel
CLC-type
Gene Expression
H+-ATPase
Metal Accumulation
Vanadium
NDC
Chemistry [ 430 ]
Language
eng
Resource Type journal article
Publisher
Elsevier Science Inc
Date of Issued 2003
Rights
Copyright (c) 2003 Elsevier Science Inc
Publish Type Author’s Original
Access Rights open access
Source Identifier
[ISSN] 1096-4959
[DOI] 10.1016/S1096-4959(03)00175-1
[DOI] http://dx.doi.org/10.1016/S1096-4959(03)00175-1 isVersionOf