広島大学水畜産学部紀要 Volume 9 Issue 2
published_at 1971-03-20

Studies on Deep Freezing Preservation of Turkey Semen

七面鳥精液の凍結保存に関する研究
Watanabe Moriyuki
Kato Shuichi
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JFacFishAnim_9_133.pdf
Abstract
5%,7%および9%ブドウ糖液を希釈液とした場合の七面鳥精子の融解後の活力および頸曲り異常精子の出現率について調べた結果は次の如くである.

1. 5%,7%および9%ブドウ糖液で4倍に希釈し急速凍結法によって凍結後融解した精子の活力割合(++以上)はそれぞれ81.5%,75%および23.9%で5%,7%ブドウ糖液を使用した精液の間にはそれほど著明な差異は認められなかったが,9%ブドウ糖液を使用した場合には前二者の場合にくらべて融解後の精子活力は著しく低下した。

2. 上記5%,7%および9%ブドウ糖液使用による融解後の頸曲り異常精子の出現率は対照の原精液の6.1%に比較しそれぞれ31.5%,14.3%,53.7%で七面鳥精液の凍結用希釈液としては7%ブドウ糖液が好適のように思われる.
3. この凍結融解後の精子活力および頸曲り異常精子出現率から7%ブドウ糖液が七面鳥精液の凍結用希釈液としてその受精率を高める上に役立つものと思われる.
Abstract
The effect of three different levels of glucose solution as a component of deep freezing diluent on the motility, per cent neck-bending spermatozoa of turkey semen was studied.

The percentages of motile spermatozoa (over ++) in thawed semen diluted with 5 per cent, 7 per cent and 9 per cent glucose solutions were on an average of 81.5 per cent, 75.0 per cent and 23.9 per cent respectively. The percentages of abnormal spermatozoa including neck-bending ones in thawed semen diluted with 5 per cent, 7 per cent and 9 per cent glucose solutions were on an average of 31.5 per cent, 14.3 per cent and 53.7 per cent. From the two view points of motility after freezing and the appearance of abnormal spermatozoa including neck-bending ones, 7 per cent glucose solution seems to be more suitable for the freezing diluent of turkey semen than 5 and 9 per cent glucose solutions.