In the previous survey of this series') in which the distribution of Vibrio parahaemolyticus (FUJINO et al. 1951) SAKAZAKI et al. 1963 in the Seto Inland Sea in summer was investigated, the biotype 1 organisms of the bacterium were isolated at high ratio from the sandy sea bottoms.
A similar survey was carried out in the central part of the Sea in March of 1964. The location of sampling stations is shown in Fig. 1 and Table 1. Biotype 1 (abbreviated I in Tables 2 and 3) were detected in the bottom samples from St. D and St. I, biotype 2 (abbreviated II in the Tables) were detected in the bottom samples from St. F and St. M and in the sea waters from St. L, St. N, and St. P. As is seen in Table 2, biotype III, an allied species, were detected only in one case, i. e., from the bottom sample of St. K. Detected frequencies of the organisms were very few, but as listed in Table 3, the results of the present and the previous surveys resembled each other in the tendency of the detected frequency.
It was interesting to note that the biotype 1 organisms were found in sandy sea bottom in winter as well as in summer.