Freezing of Fowl Semen (Gallus domesticus)

鶏精子の凍結保存試験に15ヵ月令のLeghorn種,　New　Hampshire種およびNH×Lの雄鶏11羽の精液を又凍結精液をもってする人工授精には21羽のWhite　Leghorn種を使用した。凍結はポーランドのLada-Gorzowskaら(1975)の方法によって実施した。この方法の要点は次の3つにある。(1) 採取精液は－20℃までは除々に下げてゆきその後－196℃の液体窒素中に凍結する。(2) 凍結精液は融解後直ちにglycerolを除去しこれにLake氏液を数回に分けて添加して授精する。(3) 人工援精部位はcloacaより凡そ4㎝内部にあるsperm-host　gland附近の膣内に注入する。

その結果受精率は53.1～63.6%,平均56.7%でかなり良好な結果を示した。

11 Leghorn, New Hampshire and New Hampshire X Legnorn cocks, aged 15 months, as well as 23 White Leghorn hens were used in the examination of deep freezing preservation of fowl spermatozoa. The semen was processed and frozen by Lake's method modified by Lada-Gorzowska et al. (1975). These procedures included three principal points:
(1) The temperature of semen samples was gradually reduced to －20°C before the deep freezing in liquid nitrogen (－196°C).
(2) Before insemination, the glycerol was removed from the semen by centrifuging a 700 gl at 5°C and a non-glycerol diluent by Lake was added.
(3) Artificial insemination was intra-vaginal (about 4 cm depth), estimated to be in the vicinity of the sperm-host glands.

The average percentage of fertile eggs following single inseminations in three trials was 56.7 percent, ranging from 53.1 to 63.6 percent.