Fertility of Turkey Spermatozoa Frozen for Two Years

鶏精子の長期凍結保存に引きつづき，マッサージ法によって採取した七面鳥精子をグリセリンの最終濃度7%の5.5%ブドウ糖・卵黄希釈液で4倍に希釈し，急速法によって-196℃の液体窒素中に638日～739日間凍結保存した精子の融解後の活力および同精液使用による受精率を調べた結果は次の如く要約される。

1. 液体窒素中に638日，642日，643日および739日間凍結保存した七面鳥精子の融解後の活力は80～95%，平均87.5%(++以上)で，長期保存により精子活力が著しい影響を受けるとは思われない.
2. 同精液使用による注入後3週間の受精率は3.8%であった.

Turkey semen diluted with a 6.5% glucose egg yolk solution containing 7 % glycerol in the final concentration and quickly frozen by liquid nitrogen was stored at -196℃ for 638 to 739 days. Motility and fertility of spermatozoa in the thawed semen were studied. The following results were obtained.

1. Percentage of motile spermatozoa in the thawed semen stored for 638, 642, 643 and 739 days was on average 87.5% (over ++) ranging between 80 and 95%. Spermatozoa motility did not appear to be adversely affected by the long storage time.
2. Fertility for three weeks following insemination was 3.8%.