アワビ属精液の凍結保存に関する研究 : 予報

Several experiments were preliminarily performed to find out a simple and sure cryopreserving method of abalone sperm. Using glycerol as a cryoprotectant, abalone sperm was successfully cryopressrved. The highest percentage of eggs which developed to the veliger stage from those inseminated with cryopreserved sperm was 93%. The motility of sperm which was cryopreserved by using DMSO as a cryolsrotectant was higher than that of sperm presseved with glycerol, though the percentage of larvae developing from eegs inseminated with DMSO preserved sperm was lower. Througth the insemination tests of fresh sperm in sea water containing DMSO and subsequent microscopical observation, it was inferred that the low fertility of sperm cryopreserved with DMSO was caused by some harmfull effects of DMSO on the spermatozoa or eggs somewhere in thc process between the acrosome reaction of spermatozoon and the first cleavage of the egg.