Mt. Nekoyama, located in Shobara City, is one of the rare serpentine areas in Hiroshima Prefecture. Plants unique to serpentine soils grow in the grasslands and rocky areas around the summit. For this reason, researchers have conducted floral surveys here for years. However, there have only been a few reports in recent years, and the flora’s current status is unknown. Thus, we surveyed the serpentine flora on Mt. Nekoyama from 2021 to 2022. The presence of most of the plants recorded in the past was confirmed. These included endangered plants characteristic of serpentinite areas, such as Saussurea modesta Kitam. and Thymus quinquecostatus Celak. var. ibukiensis (Kudô) H. Hara. However, the range of serpentinite plants was particularly limited to grasslands and rocky areas. Long-term conservation measures are necessary considering the effects of succession, climate change, and human disturbance.