Trans-differentiation of a Duodenal Phenotype on Duodenal Transplantation of Different Normal Tissues in F344 Rats
Hiroshima Journal of Medical Sciences Volume 60 Issue 1
Page 1-6
published_at 2011-03
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Title ( eng ) |
Trans-differentiation of a Duodenal Phenotype on Duodenal Transplantation of Different Normal Tissues in F344 Rats
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Creator |
Kominami Yoko
Gon Rina
Hayashi Masaomi
Nishiki Masayo
Sasaki Atsushi
Shiraishi Miho
Ueda Shinobu
Ochiya Takahiro
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Source Title |
Hiroshima Journal of Medical Sciences
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Volume | 60 |
Issue | 1 |
Start Page | 1 |
End Page | 6 |
Journal Identifire |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
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Abstract |
The mechanisms regulating stem cell differentiation and self-renewal are largely unknown. Our ultimate goal is to be able to regulate somatic stem cell differentiation and proliferation, In the present study the ability of trans-differentiation was studied when different normal tissue types were transplanted into the duodenum in rats. Pieces of ear (skin), bladder, trachea, diaphragm, pyloric gland. and forestomach from 8-week old GFP-F344 rats were transplanted into the duodenum of F344 rats. Goblet cells with alcian-blue PAS positive mucin and brash border with alkaline phosphatase (ALP) activity appeared in tissues implanted into the duodenum. In addition, GFP-positive duodenal mucosa was observed in all cases by immunohistochemical staining. Moreover, the GFP-positive cells were found to carry the GFP transgene by PCR analysis. indicating that the bladder, trachea. ear (skin), diaphragm, pyloric gland, and farestomach tissues showed a multipotential ability for differentiation. These results indicated that stem cells within tissues have a multipotential ability, trans-differentiating into different organs when transplanted into different environments.
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Keywords |
Transplantation
Hetero-tissues
Duodenum
F344 GFP Rats
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NDC |
Medical sciences [ 490 ]
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Language |
eng
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Resource Type | departmental bulletin paper |
Publisher |
Hiroshima University Medical Press
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Date of Issued | 2011-03 |
Publish Type | Version of Record |
Access Rights | open access |
Source Identifier |
[ISSN] 0018-2052
[NCID] AA00664312
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