Trans-differentiation of a Duodenal Phenotype on Duodenal Transplantation of Different Normal Tissues in F344 Rats

Watanabe Hiromitsu

Kominami Yoko

Gon Rina

Hayashi Masaomi

Nishiki Masayo

Sasaki Atsushi

Shiraishi Miho

Ueda Shinobu

Ochiya Takahiro

Kamiya Kenji

Hiroshima Journal of Medical Sciences

[PISSN] 0018-2052

[EISSN] 2433-7668

[NCID] AA00664312

The mechanisms regulating stem cell differentiation and self-renewal are largely unknown. Our ultimate goal is to be able to regulate somatic stem cell differentiation and proliferation, In the present study the ability of trans-differentiation was studied when different normal tissue types were transplanted into the duodenum in rats. Pieces of ear (skin), bladder, trachea, diaphragm, pyloric gland. and forestomach from 8-week old GFP-F344 rats were transplanted into the duodenum of F344 rats. Goblet cells with alcian-blue PAS positive mucin and brash border with alkaline phosphatase (ALP) activity appeared in tissues implanted into the duodenum. In addition, GFP-positive duodenal mucosa was observed in all cases by immunohistochemical staining. Moreover, the GFP-positive cells were found to carry the GFP transgene by PCR analysis. indicating that the bladder, trachea. ear (skin), diaphragm, pyloric gland, and farestomach tissues showed a multipotential ability for differentiation. These results indicated that stem cells within tissues have a multipotential ability, trans-differentiating into different organs when transplanted into different environments.

Transplantation

Hetero-tissues

Duodenum

F344 GFP Rats

医学 [ 490 ]

英語

Hiroshima University Medical Press

[ISSN] 0018-2052

[NCID] AA00664312