Thermal Structure Transformation of Methylammonium Vanadate and it's Application as a Negative Staining Reagent for Observing SARS-CoV-2

Sukmana Ndaru Candra

Sugiarto

Shinogi Jun

Yamamoto Akima

Higashiura Akifumi

Sakaguchi Takemasa

Sadakane Masahiro

European Journal of Inorganic Chemistry

The solid-state thermal structure transformation of methylammonium vanadate, (CH3NH3)VO3, from −150 °C to 350 °C is reported. Variable-temperature X-ray single-crystal structure analysis at 23, 0, −50, −100, and −150 °C reveal (CH3NH3)VO3 comprises of methylammonium cations and “snake-like” ([VO3]−)n anion chains propagating along the c-direction in the Pna21 space group. In between −150 and −100 °C, we observe a reversible structural transformation due to the re-orientation of the methylammonium cations in the crystal packing, which is also confirmed by the reversible profiles observed in differential scanning calorimetry. The methylammonium vanadate is stable until at ca. 100 °C and further heating releases methylamine and water and V2O5 is formed at ca. 275 °C . Furthermore, we show that the methylammonium vanadate can be used as a negative staining reagent for visualizing SARS-CoV-2, allowing us to discern the spike proteins from the body of the virus using transmission electron microscopy.

Negative staining reagents

Polyoxometalates

Polyoxovanadates

Structure elucidation

Thermochemistry

英語

Wiley

This is the peer reviewed version of the following article: N. C. Sukmana Sugiarto, J. Shinogi, A. Yamamoto, A. Higashiura, T. Sakaguchi, M. Sadakane, Eur. J. Inorg. Chem. 2022, e202200322., which has been published in final form at https://doi.org/10.1002/ejic.202200322. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.

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[DOI] https://doi.org/10.1002/ejic.202200322 ～の異版である