MicroRNA-26a deficiency attenuates the severity of frozen shoulder in a mouse immobilization model

Sumimoto Yasuhiko

Harada Yohei

Yimiti Dilimulati

Watanabe Chikara

Miyaki Shigeru

Adachi Nobuo

Journal of Orthopaedic Research

The main pathogenesis of the frozen shoulder is thought to be the inflammation of the intra-articular synovium and subsequent fibrosis of the shoulder joint capsule. However, the molecular pathogenesis of the frozen shoulder is still unknown. A class of noncoding RNAs, microRNAs contribute to various diseases including musculoskeletal diseases. MicroRNA-26a (miR-26a) has been reported to be associated with fibrosis in several organs. This study aims to reveal the role of miR-26a on fibrosis in the shoulder capsule using a frozen shoulder model in miR-26a deficient (miR-26a KO) mice. MiR-26a KO and wild-type (WT) mice were investigated using a frozen shoulder model. The range of motion (ROM) of the shoulder, histopathological changes such as synovitis, and fibrosis-related gene expression in the model mice were evaluated to determine the role of miR-26a. In WT mice, both inflammatory cell infiltration and thickening of the inferior shoulder joint capsule were observed after 1 week of immobilization, and this thickening further progressed over the subsequent 6 weeks. However, the immobilized shoulder in miR-26a KO mice consistently exhibited significantly better ROM compared with WT mice at 1 and 6 weeks, and histological changes were significantly less severe. The expression of inflammation- and fibrosis-related genes was decreased in the miR-26a KO mice compared with WT mice at 1 and 6 weeks. Together, miR-26a deficiency attenuated the severity of frozen shoulder in the immobilization model mouse. The present study suggests that miR-26a has the potential to be a target miRNA for therapeutic approach to frozen shoulder.

fibrosis

frozen shoulder

inflammation

knockout mice

microRNA-26a

eng

Wiley

This is the peer reviewed version of the following article: Journal of Orthopaedic Research, 42(12), 2623-2633, MicroRNA-26a deficiency attenuates the severity of frozen shoulder in a mouse immobilization model, Yasuhiko Sumimoto, Yohei Harada, Dilimulati Yimiti, Chikara Watanabe, Shigeru Miyaki, Nobuo Adachi, which has been published in final form at https://doi.org/10.1002/jor.25940. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.

This is not the published version. Please cite only the published version.

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[DOI] https://doi.org/10.1002/jor.25940 isVersionOf

日本学術振興会

Japan Society for the Promotion of Science

[Crossref Funder] https://doi.org/10.13039/501100001691

microRNAを標的とした凍結肩の病態解明と新たな治療戦略

microRNAを標的とした凍結肩の病態解明と新たな治療戦略

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