Periostin regulates integrin expression in gingival epithelial cells

Journal of Oral Biosciences Volume 66 Issue 1 Page 170-178 published_at 2023-12-03
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Title ( eng )
Periostin regulates integrin expression in gingival epithelial cells
Creator
Reika Hirata
Nagahara Takayoshi
Kudo Yasusei
Source Title
Journal of Oral Biosciences
Volume 66
Issue 1
Start Page 170
End Page 178
Abstract
Objective
Human gingival epithelial cells (HGECs) function as a mechanical barrier against invasion by pathogenic organisms through epithelial cell–cell junction complexes, which are complex components of integrin. Integrins play an important role in the protective functions of HGECs. Human periodontal ligament (HPL) cells regulate periodontal homeostasis. However, periodontitis results in the loss of HPL cells. Therefore, as replenishment, HPL cells or mesenchymal stem cells (MSCs) can be transplanted. Herein, HPL cells and MSCs were used to elucidate the regulatory mechanisms of HGECs, assuming periodontal tissue homeostasis.
Methods
Human gingival fibroblasts (HGFs), HGECs, HPL cells, and MSCs were cultured, and the conditioned medium was collected. With or without silencing periostin mRNA, HGECs were cultured under normal conditions or in a conditioned medium. Integrin and periostin mRNA expression was determined using real-time polymerase chain reaction. Integrin protein expression was analyzed using flow cytometry, and periostin protein expression was determined via western blotting.
Results
The conditioned medium affected integrin expression in HGECs. Higher expression of periostin was observed in MSCs and HPL cells than in HGFs. The conditioned medium that contained periostin protein regulated integrin expression in HGECs. After silencing periostin in MSCs and HPL cells, periostin protein was not detected in the conditioned medium, and integrin expression in HGECs remained unaffected.
Conclusions
Integrins in HGECs are regulated by periostin secreted from HPL cells and MSCs. This result suggests that periostin maintains gingival cell adhesion and regulates bacterial invasion/infection. Therefore, the functional regulation of periostin-secreting cells is important in preventing periodontitis.
Keywords
Gingival epithelial cells
Mesenchymal stem cells
Human periodontal ligament cells
Periostin
Integrin beta
Language
eng
Resource Type journal article
Publisher
Elsevier
Date of Issued 2023-12-03
Rights
© 2023. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
This is not the published version. Please cite only the published version.
この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。
Publish Type Accepted Manuscript
Access Rights open access
Source Identifier
[DOI] https://doi.org/10.1016/j.job.2023.11.009 isVersionOf