High-Density Protein Loading on Hierarchically Porous LDH-Aluminum Hydroxide Composites with a Rational Mesostructure

Langmuir Volume 32 Issue 35 Page 8826-8833 published_at 2016-08-08
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Title ( eng )
High-Density Protein Loading on Hierarchically Porous LDH-Aluminum Hydroxide Composites with a Rational Mesostructure
Tokudome Yasuaki
Fukui Megu
Nishimura Sari
Prevot Vanessa
Forano Claude
Poologasundarampillai Gowsihan
Lee Peter D.
Takahashi Masahide
Source Title
Volume 32
Issue 35
Start Page 8826
End Page 8833
Hierarchically porous biocompatible Mg-Al-Cl type LDH composites containing aluminum hydroxide (Alhy) have been prepared using a phase-separation process. The sol-gel synthesis allows for the hierarchical pores of the LDH-Alhy composites to be tuned, leading to a high specific solid surface area per unit volume available for high molecular weight protein adsorptions. A linear relationship between effective surface area, SEFF, and loading capacity of a model protein, bovine serum albumin (BSA) is established following successful control of the structure of the LDH-Alhy composite. The threshold of mean pore diameter, Dpm, above which BSA is effectively adsorbed on the surface of LDH-Alhy composites, is deduced as 20 nm. In particular, LDH-Alhy composite aerogels obtained via supercritical drying exhibits extremely high capacity for protein loading (996 mg/g) due to a large mean mesopore diameter (> 30 nm). The protein loading on LDH-Alhy is >14 times that of a reference LDH material (70 mg/g) prepared via a standard procedure. Importantly, BSA molecules pre-adsorbed on porous composites were successfully released on soaking in ionic solutions (HPO42− and Cl− aq.). The superior capability of the biocompatible LDH materials for loading, encapsulation, and releasing large quantity of proteins was clearly demonstrated, which potential uses in separation and purification in addition to a high-capacity storage medium.
The present work is supported by JSPS-MAE SAKURA program (N°34148TB).
The present work is partially supported by JSPS KAKENHI, and by a research grant from the Foundation for the Promotion of Ion Engineering.
Resource Type journal article
American Chemical Society
Date of Issued 2016-08-08
© 2016 American Chemical Society
This document is the Accepted Manuscript version of a Published Work that appeared in final form in Langmuir, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.langmuir.6b01925
This is not the published version. Please cite only the published version. この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。
Publish Type Author’s Original
Access Rights open access
Source Identifier
[ISSN] 1520-5827
[NCID] AA10461730
[DOI] 10.1021/acs.langmuir.6b01925
[DOI] https://doi.org/10.1021/acs.langmuir.6b01925