Effects of S-glutathionylation on the passive force–length relationship in skeletal muscle fibres of rats and humans

Journal of Muscle Research and Cell Motility 41 巻 239-250 頁 2019-11-02 発行
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タイトル ( eng )
Effects of S-glutathionylation on the passive force–length relationship in skeletal muscle fibres of rats and humans
作成者
Lamboley Cedric R.
Lamb Graham D.
収録物名
Journal of Muscle Research and Cell Motility
41
開始ページ 239
終了ページ 250
抄録
This study investigated the efect of S-glutathionylation on passive force in skeletal muscle fbres, to determine whether activity-related redox reactions could modulate the passive force properties of muscle. Mechanically-skinned fbres were freshly obtained from human and rat muscle, setting sarcomere length (SL) by laser difraction. Larger stretches were required to produce passive force in human fbres compared to rat fbres, but there were no fbre-type diferences in either species. When fbres were exposed to glutathione disulfde (GSSG; 20 mM, 15 min) whilst stretched (at a SL where passive force reached~20% of maximal Ca2+-activated force, denoted as SL20 % max), passive force was subsequently decreased at all SLs in both type I and type II fbres of rat and human (e.g., passive force at SL20 % max decreased by 12 to 25%). This decrease was fully reversed by subsequent reducing treatment with dithiothreitol (DTT; 10 mM for 10 min). If freshly skinned fbres were initially treated with DTT, there was an increase in passive force in type II fbres (by 10±3% and 9±2% in rat and human fbres, respectively), but not in type I fbres. These results indicate that (i) S-glutathionylation, presumably in titin, causes a decrease in passive force in skeletal muscle fbres, but the reduction is relatively smaller than that reported in cardiac muscle, (ii) in rested muscle in vivo, there appears to be some level of reversible oxidative modifcation, probably involving S-glutathionylation of titin, in type II fbres, but not in type I fbres.
著者キーワード
Oxidative stress
Muscle elasticity
Skinned fbre
Titin
Passive force
内容記述
We thank the National Health and Medical Research Council of Australia for financial support (Grant No. 1085331).
言語
英語
資源タイプ 学術雑誌論文
出版者
Springer
発行日 2019-11-02
権利情報
This is not the published version. Please cite only the published version. この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。
出版タイプ Author’s Original(十分な品質であるとして、著者から正式な査読に提出される版)
アクセス権 オープンアクセス
収録物識別子
[ISSN] 0142-4319
[ISSN] 1573-2657
[DOI] 10.1007/s10974-019-09563-5
[DOI] https://doi.org/10.1007/s10974-019-09563-5