Use of the Fluorescent Staining Method for Determining the Viability of Mycobacterium lepraemurium
Hiroshima Journal of Medical Sciences Volume 34 Issue 1
Page 161-163
published_at 1985-03
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| File | |
| Title ( eng ) |
Use of the Fluorescent Staining Method for Determining the Viability of Mycobacterium lepraemurium
|
| Creator |
TSUKIYAMA Fumiaki
KATOH Masafumi
NOMURA Toshiya
MATSUO Yoshiyasu
|
| Source Title |
Hiroshima Journal of Medical Sciences
|
| Volume | 34 |
| Issue | 1 |
| Start Page | 161 |
| End Page | 163 |
| Journal Identifire |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
|
| Abstract |
The cell suspension of Mycobacterium lepraemurium was exposed to heating of 40°C to 70°C for various lengths of time. The percent green-fluorescent cell by the midified fluorescein diacetate (FDA) -ethidium bromide (EB) staining method was calculated and compared with the infectivity to mice. The reduction in the percentage was associated significantly with the loss of the infectivity.
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| Keywords |
Mycobacterium lepraemurium
FDA/EB staining
Viability
|
| NDC |
Medical sciences [ 490 ]
|
| Language |
eng
|
| Resource Type | departmental bulletin paper |
| Publisher |
Hiroshima University School of Medicine
|
| Date of Issued | 1985-03 |
| Publish Type | Version of Record |
| Access Rights | open access |
| Source Identifier |
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 2410394
|
