Production of Interleukin 2 in Human Peripheral Blood Lymphocytes: Optimal Condition for its Culture
Hiroshima Journal of Medical Sciences Volume 34 Issue 1
Page 43-51
published_at 1985-03
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| File | |
| Title ( eng ) |
Production of Interleukin 2 in Human Peripheral Blood Lymphocytes: Optimal Condition for its Culture
|
| Creator |
YAMAKIDO Michio
YANAGIDA Jitsuro
ISHIOKA Sinichi
MATSUZAKA Shigeru
HOZAWA Soichiro
AKIYAMA Mitoshi
KOBUKE Kyoko
INAMIZU Tsutomu
NISHIMOTO Yukio
|
| Source Title |
Hiroshima Journal of Medical Sciences
|
| Volume | 34 |
| Issue | 1 |
| Start Page | 43 |
| End Page | 51 |
| Journal Identifire |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
|
| Abstract |
For the assay of the production of Interleukin 2 (IL-2) in human peripheral blood lymphocytes (human PBL), a study was made on the optimal condition for its culture.
1) The optimal condition for the production of IL-2 was considered to be incubation time of 24 hr, number of PBL of 1 x 106 cells/ml, and phytohemagglutinin-M (PHA-M) concentration of 1 — 5% 2) By one way mixed lymphocyte reaction (one way MLR), IL-2 activity similar in level to that by PHA-M stimulation could also be obtained. It was maximal at the 4th day of culture. 3) By PHA-P (0.06%) stimulation, IL-2 could also be produced similar to that by PHAM stimulation but it decreased in the order of Con A, PWM and PPD and hardly any production of IL-2 could be observed by LPS. |
| Keywords |
Production of Interleukin 2
Human peripheral blood lymphocytes
Optimal condition
CTLL-2 microassay
|
| NDC |
Medical sciences [ 490 ]
|
| Language |
eng
|
| Resource Type | departmental bulletin paper |
| Publisher |
Hiroshima University School of Medicine
|
| Date of Issued | 1985-03 |
| Publish Type | Version of Record |
| Access Rights | open access |
| Source Identifier |
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 3874854
|
