RSC Chromatin-Remodeling Complex Is Important for Mitochondrial Function in Saccharomyces cerevisiae

PLoS ONE Volume 10 Issue 6 Page e0130397- published_at 2015-06-18
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Title ( eng )
RSC Chromatin-Remodeling Complex Is Important for Mitochondrial Function in Saccharomyces cerevisiae
Creator
Imamura Yuko
Yu Feifei
Nakamura Misaki
Chihara Yuhki
Okane Kyo
Sato Masahiro
Kanai Muneyoshi
Hamada Ryoko
Source Title
PLoS ONE
Volume 10
Issue 6
Start Page e0130397
Abstract
RSC (Remodel the Structure of Chromatin) is an ATP-dependent chromatin remodeling complex essential for the growth of Saccharomyces cerevisiae. RSC exists as two distinct isoforms that share core subunits including the ATPase subunit Nps1/Sth1 but contain either Rsc1or Rsc2. Using the synthetic genetic array (SGA) of the non-essential null mutation method, we screened for mutations exhibiting synthetic growth defects in combination with the temperature-sensitive mutant, nps1-105, and found connections between mitochondrial function and RSC. rsc mutants, including rsc1Δ, rsc2Δ, and nps1-13, another temperature-sensitive nps1 mutant, exhibited defective respiratory growth; in addition, rsc2Δ and nps1-13 contained aggregated mitochondria. The rsc2Δ phenotypes were relieved by RSC1 overexpression, indicating that the isoforms play a redundant role in respiratory growth. Genome-wide expression analysis in nps1-13 under respiratory conditions suggested that RSC regulates the transcription of some target genes of the HAP complex, a transcriptional activator of respiratory gene expression. Nps1 physically interacted with Hap4, the transcriptional activator moiety of the HAP complex, and overexpression of HAP4 alleviated respiratory defects in nps1-13, suggesting that RSC plays pivotal roles in mitochondrial gene expression and shares a set of target genes with the HAP complex.
Descriptions
This study was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan to ET (22580086), and MY (19780076); URL: http://www.jsps.go.jp/j-grantsinaid/index.html.
Language
eng
Resource Type journal article
Publisher
Public Library of Science
Date of Issued 2015-06-18
Rights
© 2015 Imamura et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Publish Type Version of Record
Access Rights open access
Source Identifier
[ISSN] 1932-6203
[DOI] 10.1371/journal.pone.0130397
[DOI] https://doi.org/10.1371/journal.pone.0130397