Constant Enthalpy Change Value during Pyrophosphate Hydrolysis within the Physiological Limits of NaCl
Journal of Biological Chemistry 288 巻 41 号
29247-29251 頁
2013-10-11 発行
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| ファイル情報(添付) | |
| タイトル ( eng ) |
Constant Enthalpy Change Value during Pyrophosphate Hydrolysis within the Physiological Limits of NaCl
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| 作成者 |
Wakai Satoshi
Kidokoro Shun-ichi
Masaki Kazuo
Nakasone Kaoru
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| 収録物名 |
Journal of Biological Chemistry
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| 巻 | 288 |
| 号 | 41 |
| 開始ページ | 29247 |
| 終了ページ | 29251 |
| 抄録 |
A decrease in water activity was thought to result in smaller enthalpy change values during PPi hydrolysis, indicating the importance of solvation for the reaction. However, the physiological significance of this phenomenon is unknown. Here, we combined biochemistry and calorimetry to solve this problem using NaCl, a physiologically occurring water activity-reducing reagent. The pyrophosphatase activities of extremely halophilic Haloarcula japonica, which can grow at ∼4 m NaCl, and non-halophilic Escherichia coli and Saccharomyces cerevisiae were maximal at 2.0 and 0.1 m NaCl, respectively. Thus, halophilic and non-halophilic pyrophosphatases exhibit distinct maximal activities at different NaCl concentration ranges. Upon calorimetry, the same exothermic enthalpy change of −35 kJ/mol was obtained for the halophile and non-halophiles at 1.5–4.0 and 0.1–2.0 m NaCl, respectively. These results show that solvation changes caused by up to 4.0 m NaCl (water activity of ∼0.84) do not affect the enthalpy change in PPi hydrolysis. It has been postulated that PPi is an ATP analog, having a so-called high energy phosphate bond, and that the hydrolysis of both compounds is enthalpically driven. Therefore, our results indicate that the hydrolysis of high energy phosphate compounds, which are responsible for biological energy conversion, is enthalpically driven within the physiological limits of NaCl.
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| 内容記述 |
This work was supported by Grant-in-aid for Scientific Research on Innovative Areas 20118005 from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
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| 言語 |
英語
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| 資源タイプ | 学術雑誌論文 |
| 出版者 |
The American Society for Biochemistry and Molecular Biology, Inc.
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| 発行日 | 2013-10-11 |
| 権利情報 |
This research was originally published in the Journal of Biological Chemistry. Satoshi Wakai, Shun-ichi Kidokoro, Kazuo Masaki, Kaoru Nakasone, and Yoshihiro Sambongi. Constant Enthalpy Change Value during Pyrophosphate Hydrolysis within the Physiological Limits of NaCl. J Biol Chem. 2013; 288:29247-29251. © 2013 the American Society for Biochemistry and Molecular Biology, Inc.
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| 出版タイプ | Version of Record（出版社版。早期公開を含む） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
[ISSN] 0021-9258
[ISSN] 1083-351X
[DOI] 10.1074/jbc.M113.502963
[DOI] https://doi.org/10.1074/jbc.M113.502963
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