The development of fluorescent protein tracing vectors for multicolor imaging of clinically isolated Staphylococcus aureus
Scientific Reports Volume 7
Page 2865-
published_at 2017-06-06
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Title ( eng ) |
The development of fluorescent protein tracing vectors for multicolor imaging of clinically isolated Staphylococcus aureus
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Creator |
Nakamura Motoki
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Source Title |
Scientific Reports
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Volume | 7 |
Start Page | 2865 |
Abstract |
Recent advances in fluorescent protein technology provide a wide variety of biological imaging applications; however current tools for bio-imaging in the Gram-positive bacterium Staphylococcus aureus has necessitated further developments for fluorescence intensity and for a multicolor palette of fluorescent proteins. To enhance the expression of multicolor fluorescent proteins in clinical S. aureus strains, we developed new fluorescent protein expression vectors, containing the blaZ/sodp promoter consisting of the β-lactamase gene (blaZ) promoter and the ribosome binding site (RBS) of superoxide dismutase gene (sod). We found S. aureus-adapted GFP (GFPsa) driven by the blaZ/sodp promoter was highly expressed in the S. aureus laboratory strain RN4220, but not in the clinical strains, MW2 and N315, harboring the endogenous blaI gene, a repressor of the blaZ gene promoter. We therefore constructed a constitutively induced blaZ/sodp promoter (blaZ/sodp(Con)) by introducing substitution mutations into the BlaI binding motif, and this modification allowed enhanced expression of the multicolor GFP variants (GFPsa, EGFP, mEmerald, Citrine, Cerulean, and BFP) as well as codon-optimized reef coral fluorescent proteins (mCherry and AmCyan) in the S. aureus clinical strains. These new fluorescent probes provide new tools to enhance expression of multicolor fluorescent proteins and facilitate clear visualization of clinical S. aureus strains.
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Descriptions |
This study was supported by Grant-in-Aid for Young Scientists (B) Grant Number JP25861744 and Grant-in-Aid for Scientific Research (C) Grant Number JP25460533 from the Japan Society for the promotion of Science (JSPS). A confocal laser scanning microscopy was performed at the Analysis Center of Life Science, Natural Science Center for Basic Research and Development, Hiroshima University.
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Language |
eng
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Resource Type | journal article |
Publisher |
Nature Research
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Date of Issued | 2017-06-06 |
Rights |
© The Author(s) 2017. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
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Publish Type | Version of Record |
Access Rights | open access |
Source Identifier |
[ISSN] 2045-2322
[DOI] 10.1038/s41598-017-02930-7
[PMID] 28588310
[DOI] https://doi.org/10.1038/s41598-017-02930-7
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