Two spatially distinct kinesin-14 proteins, Pkl1 and Klp2, generate collaborative inward forces against kinesin-5 Cut7 in S. pombe
Journal of Cell Science 131 巻 1 号
jcs210740- 頁
2018-01-04 発行
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| ファイル情報(添付) | |
| タイトル ( eng ) |
Two spatially distinct kinesin-14 proteins, Pkl1 and Klp2, generate collaborative inward forces against kinesin-5 Cut7 in S. pombe
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| 作成者 |
Yamada Yusuke
Yamauchi Tomoaki
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| 収録物名 |
Journal of Cell Science
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| 巻 | 131 |
| 号 | 1 |
| 開始ページ | jcs210740 |
| 抄録 |
Kinesin motors play central roles in bipolar spindle assembly. In many eukaryotes, spindle pole separation is driven by kinesin-5, which generates outward force. This outward force is balanced by antagonistic inward force elicited by kinesin-14 and/or dynein. In fission yeast, two kinesin-14 proteins, Pkl1 and Klp2, play an opposing role against the kinesin-5 motor protein Cut7. However, how the two kinesin-14 proteins coordinate individual activities remains elusive. Here, we show that although deletion of either pkl1 or klp2 rescues temperature-sensitive cut7 mutants, deletion of only pkl1 can bypass the lethality caused by cut7 deletion. Pkl1 is tethered to the spindle pole body, whereas Klp2 is localized along the spindle microtubule. Forced targeting of Klp2 to the spindle pole body, however, compensates for Pkl1 functions, indicating that cellular localizations, rather than individual motor specificities, differentiate between the two kinesin-14 proteins. Interestingly, human kinesin-14 (KIFC1 or HSET) can replace either Pkl1 or Klp2. Moreover, overproduction of HSET induces monopolar spindles, reminiscent of the phenotype of Cut7 inactivation. Taken together, this study has uncovered the biological mechanism whereby two different Kinesin- 14 motor proteins exert their antagonistic roles against kinesin-5 in a spatially distinct manner.
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| 著者キーワード |
Fission yeast
Force generation
Kinesin
Mitotic bipolar spindle
Spindle pole body
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| 内容記述 |
This work was supported by the Japan Society for the Promotion of Science (JSPS) [KAKENHI Scientific Research (A) 16H02503 to T.T., a Challenging Exploratory Research grant 16K14672 to T.T., Scientific Research (C) 16K07694 to M.Y.], the Naito Foundation (T.T.) and the Uehara Memorial Foundation (T.T).
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| 言語 |
英語
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| 資源タイプ | 学術雑誌論文 |
| 出版者 |
The Company of Biologists Ltd
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| 発行日 | 2018-01-04 |
| 権利情報 |
© 2018. Published by The Company of Biologists Ltd
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| 出版タイプ | Version of Record（出版社版。早期公開を含む） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
[ISSN] 0021-9533
[ISSN] 1477-9137
[DOI] 10.1242/jcs.210740
[PMID] 29167352
[DOI] https://doi.org/10.1242/jcs.210740
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