The genome sequence of Streptomyces rochei 7434AN4, which carries a linear chromosome and three characteristic linear plasmids
Scientific Reports Volume 9
Page 10973-
published_at 2019-07-29
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| File | |
| Title ( eng ) |
The genome sequence of Streptomyces rochei 7434AN4, which carries a linear chromosome and three characteristic linear plasmids
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| Creator |
Nindita Yosi
Cao Zhisheng
Fauzi Amirudin Akhmad
Teshima Aiko
Misaki Yuya
Muslimin Rukman
Yang Yingjie
Shiwa Yuh
Yoshikawa Hirofumi
Tagami Michihira
Lezhava Alexander
Ishikawa Jun
Kuroda Makoto
Sekizuka Tsuyoshi
Kinashi Haruyasu
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| Source Title |
Scientific Reports
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| Volume | 9 |
| Start Page | 10973 |
| Abstract |
Streptomyces rochei 7434AN4 produces two structurally unrelated polyketide antibiotics, lankacidin and lankamycin, and carries three linear plasmids, pSLA2-L (211 kb), -M (113 kb), and -S (18 kb), whose nucleotide sequences were previously reported. The complete nucleotide sequence of the S. rochei chromosome has now been determined using the long-read PacBio RS-II sequencing together with short-read Illumina Genome Analyzer IIx sequencing and Roche 454 pyrosequencing techniques. The assembled sequence revealed an 8,364,802-bp linear chromosome with a high G + C content of 71.7% and 7,568 protein-coding ORFs. Thus, the gross genome size of S. rochei 7434AN4 was confirmed to be 8,706,406 bp including the three linear plasmids. Consistent with our previous study, a tap-tpg gene pair, which is essential for the maintenance of a linear topology of Streptomyces genomes, was not found on the chromosome. Remarkably, the S. rochei chromosome contains seven ribosomal RNA (rrn) operons (16S-23S-5S), although Streptomyces species generally contain six rrn operons. Based on 2ndFind and antiSMASH platforms, the S. rochei chromosome harbors at least 35 secondary metabolite biosynthetic gene clusters, including those for the 28-membered polyene macrolide pentamycin and the azoxyalkene compound KA57-A.
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| Descriptions |
This work was supported by Grants-in-Aid for Scientific Research on Innovative Areas (23108515, 25108718 and 17H05446 to K.A.) from Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT), Grants-in-Aid for Scientific Research (B) (16H04917 to K.A.) from the Japan Society for the Promotion of Science (JSPS), and the Sasakawa Scientific Research Grant from the Japan Science Society to Y.N. This work was partly supported by a JSPS A3 Foresight Program. A.A.F. and R.M. were supported by the Indonesia Endowment Fund for Education (LPDP). Sequencing analysis using an Illumina GAIIx sequencer was supported by the Grant in Aid for Scientific Research on Innovative Areas (22108010 to J.I.) from MEXT.
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| Language |
eng
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| Resource Type | journal article |
| Publisher |
Nature Research
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| Date of Issued | 2019-07-29 |
| Rights |
© The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
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| Publish Type | Version of Record |
| Access Rights | open access |
| Source Identifier |
[ISSN] 2045-2322
[DOI] 10.1038/s41598-019-47406-y
[DOI] https://doi.org/10.1038/s41598-019-47406-y
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