Establishment of an in Vitro Peyer's Patch Cell Culture System Correlative to in Vivo Study Using Intestine and Screening of Lactic Acid Bacteria Enhancing Intestinal Immunity
Biological and Pharmaceutical Bulletin 33 巻 2 号
289-293 頁
2010-02-01 発行
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| ファイル情報(添付) | |
| タイトル ( eng ) |
Establishment of an in Vitro Peyer's Patch Cell Culture System Correlative to in Vivo Study Using Intestine and Screening of Lactic Acid Bacteria Enhancing Intestinal Immunity
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| 作成者 |
Jin Hekui
Zhao Xingrong
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| 収録物名 |
Biological and Pharmaceutical Bulletin
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| 巻 | 33 |
| 号 | 2 |
| 開始ページ | 289 |
| 終了ページ | 293 |
| 抄録 |
Some lactic acid bacteria (LAB) are known as representative of probiotics. To screen LAB effective to enhance intestinal immunity, in the present study, we developed an accurate and convenient in vitro evaluation system using Peyer's patch cells (PP-cells) isolated from the mice intestine. We observed that the amount of immunoglobulin A (IgA) produced by PP-cells co-cultured with LAB was well correlative to that in PP-cells, intestine and feces isolated from live mice after oral administration of LAB [correlation coefficient (r)=0.888, 0.883, and 0.920, respectively]. In addition, using this in vitro system, we suggest that the IgA level of PP-cells co-culturing with plant-derived LAB might be more enhanced than with animal-derived LAB.
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| 著者キーワード |
lactic acid bacterium
intestinal immunity
immunoglobulin A
probiotic
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| 言語 |
英語
|
| 資源タイプ | 学術雑誌論文 |
| 出版者 |
The Pharmaceutical Society of Japan
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| 発行日 | 2010-02-01 |
| 権利情報 |
© The Pharmaceutical Society of Japan
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| 出版タイプ | Version of Record（出版社版。早期公開を含む） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
[ISSN] 1347-5215
[ISSN] 0918-6158
[DOI] 10.1248/bpb.33.289
[DOI] https://doi.org/10.1248/bpb.33.289
[PMID] 20118555
[NCID] AA10885497
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