Development of butanol-tolerant Bacillus subtilis strain GRSW2-B1 as a potential bioproduction host
AMB Express 1 巻 1 号
10- 頁
2011-05-30 発行
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| ファイル情報(添付) | |
| タイトル ( eng ) |
Development of butanol-tolerant Bacillus subtilis strain GRSW2-B1 as a potential bioproduction host
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| 作成者 |
Kataoka Naoya
Rachadech Wanitcha
Alisa S Vangnai
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| 収録物名 |
AMB Express
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| 巻 | 1 |
| 号 | 1 |
| 開始ページ | 10 |
| 抄録 |
As alternative microbial hosts for butanol production with organic-solvent tolerant trait are in high demands, a butanol-tolerant bacterium, Bacillus subtilis GRSW2-B1, was thus isolated. Its tolerance covered a range of organic solvents at high concentration (5%v/v), with remarkable tolerance in particular to butanol and alcohol groups. It was susceptible for butanol acclimatization, which resulted in significant tolerance improvement. It has versatility for application in a variety of fermentation process because it has superior tolerance when cells were exposed to butanol either as high-density, late-exponential grown cells (up to 5%v/v) or under growing conditions (up to 2.25%v/v). Genetic transformation procedure was optimized, yielding the highest efficiency at 5.17 × 103 colony forming unit (μg DNA)-1. Gene expression could be effectively driven by several promoters with different levels, where as the highest expression was observed with a xylose promoter. The constructed vector was stably maintained in the transformants, in the presence or absence of butanol stress. Adverse effect of efflux-mediated tetracycline resistance determinant (TetL) to bacterial organic-solvent tolerance property was unexpectedly observed and thus discussed. Overall results indicate that B. subtilis GRSW2-B1 has potential to be engineered and further established as a genetic host for bioproduction of butanol.
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| 著者キーワード |
Organic-solvent tolerant bacteria
Butanol-tolerant bacteria
Heterologous gene-expression host
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| 内容記述 |
This work was the collaboration of Chulalongkorn University - Hiroshima University under the Asian Core Program (ACP) and financially supported by The Japan Society for the Promotion of Science (JSPS) and the National Research Council of Thailand (NRCT) (Bilateral Project). It was partly supported by the Thai Government Stimulus Package 2 (TKK2555) under the Project for Establishment of Comprehensive Center for Innovative Food, Health Products and Agriculture (PERFECTA).
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| 言語 |
英語
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| 資源タイプ | 学術雑誌論文 |
| 出版者 |
Springer Nature
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| 発行日 | 2011-05-30 |
| 権利情報 |
© 2011 Kataoka et al; licensee Springer. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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| 出版タイプ | Version of Record（出版社版。早期公開を含む） |
| アクセス権 | オープンアクセス |
| 収録物識別子 |
[DOI] 10.1186/2191-0855-1-10
[ISSN] 2191-0855
[PMID] 21906347
[DOI] https://doi.org/10.1186/2191-0855-1-10
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