The Effectiveness of Phaleria Macrocarpa and Chemotherapy in Increasing Caspase 3 and Apoptotic Index in Epidermoid Carcinoma

Hiroshima Journal of Medical Sciences Volume 67 Page 153-160 published_at 2018-05
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Title ( eng )
The Effectiveness of Phaleria Macrocarpa and Chemotherapy in Increasing Caspase 3 and Apoptotic Index in Epidermoid Carcinoma
Creator
Sastriawan Helmi
Arifin Muhamad Thohar
Handojo Djoko
Source Title
Hiroshima Journal of Medical Sciences
Volume 67
Start Page 153
End Page 160
Journal Identifire
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
Abstract
Chemotherapy management of epidermoid carcinoma of the skin is relatively expensive and has toxic effects on vital organs. Phaleria macrocarpa is a medicinal plant that is often used as an anti-cancer. This study aims to prove the effectiveness of the Phaleria marcocarpa extract, paclitaxel-cisplatin chemotherapy and the combination of both as a neoadjuvant to increase caspase-3 expression and apoptotic index in epidermoid carcinoma of the skin in Swiss male mice. This study used male mice of the Swiss strain, after induction of epidermoid carcinoma cells, 4 group randomization, K (control), P1 (Phaleria macrocarpa 0.0715 mg/ day), P2 (paclitaxel 175 mg/m2 and cisplatin 50 mg/m2), P3 (the combination of Phaleria macrocarpa and chemotherapy). The caspase-3 expression was examined with immunohistochemical stain and the apoptotic index examination was done by staining the Tunel. The research shows that there is an increase in caspase-3 expression; the order of the increase (from the most significant to the less significant) is P3, P2, and P1 with significant result (p<0.05) compraed to the control group. There is a significant correlation between caspase-3 expression and apoptotic index with a very strong positive correlation. It can be concluded that the Phaleria marcocarpa extract, paclitaxel-cisplatin chemotherapy and the combination of both increased the caspase-3 expression and the apoptotic index of epidermoid carcinoma cell; there was no significant differences between P1 and P2 but there was a synergy effect from the administration of P1 and P2 combination, and there was significant correlation between the caspase-3 expression and apoptotic index with a very strong positive correlation.
Keywords
Phaleria marcocarpa
caspase 3
apoptotic index
epidermoid carcinoma of the skin
NDC
Medical sciences [ 490 ]
Language
eng
Resource Type departmental bulletin paper
Publisher
Hiroshima University Medical Press
Date of Issued 2018-05
Rights
Copyright (c) 2018 Hiroshima University Medical Press
Publish Type Version of Record
Access Rights open access
Source Identifier
[ISSN] 0018-2052
[NCID] AA00664312