The Effect of Green Tea Epigallocatechin-3-Gallate on Spatial Memory Function, Malondialdehyde and TNF-α Level in D-Galactose-Induced BALB/C Mice
Hiroshima Journal of Medical Sciences Volume 67
Page 41-48
published_at 2018-05
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Title ( eng ) |
The Effect of Green Tea Epigallocatechin-3-Gallate on Spatial Memory Function, Malondialdehyde and TNF-α Level in D-Galactose-Induced BALB/C Mice
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Creator |
Gumay Ainun Rahmasari
Bakri Saekhol
Pudjonarko Dwi
Suprihati
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Source Title |
Hiroshima Journal of Medical Sciences
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Volume | 67 |
Start Page | 41 |
End Page | 48 |
Journal Identifire |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
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Abstract |
Introduction : Neuroinflammatory process and oxidative stress play an important role in the mechanism of brain aging and neurodegenerative diseases such as Alzheimer. Epigallocatechin-3-gallate (EGCG) have antioxidant, anti-inflammatory, and neuroprotective effects. This study aims to prove the effect of green tea EGCG on spatial memory function, malondialdehyde (MDA), and TNF-α level in Balb/c mice induced by D-galactose. Method : An experimental study using ”post test only control group design”. The samples were 18 male Balb/c mices, aged 6-8 weeks, divided into 3 groups. Negative control group (N-C) was induced by subcutaneous injection of D-galactose (150 mg/kg) once daily for 6 weeks. EGCG-2 and EGCG-6 were induced by D-galactose and orally administered by 2 and 6 mg/kg EGCG once daily for 5 weeks. The indicator of examination were spatial memory function using morris water maze, MDA and TNF-α level using Elisa. One-way Anova, Kruskal-Wallis, and Pearson were used for statistical analysis. Results : Means of % escape latency time and path length in the EGCG-2 {42,02(SD=5,9);43,47(SD=5,97)%} and EGCG-6 {40,45(SD=6,5); 41,78(SD=6,77)%} were significantly higher than N-C {28,68(SD=9,15), p=0,013; 32,98(SD=7,75)%,p=0,04}. MDA level in the EGCG-2 {587,79(SD=76,04)ng/ml} was significantly smaller than N-C {722,64(SD=134,78)ng/ml,p=0,037}. TNF-α level in all groups was not different (p=0,786). There was a significant and strong correlation between MDA level and spatial memory function (r=-0.551; p=0,018). Conclusion : EGCG may improve spatial memory function and oxidative stress in mice induced dementia, but it may not improve the status of neuroinflammation.
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Keywords |
Green tea epigallocatechin-3-gallate
spatial memory function
MDA
TNF- α
D- galactose
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NDC |
Medical sciences [ 490 ]
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Language |
eng
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Resource Type | departmental bulletin paper |
Publisher |
Hiroshima University Medical Press
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Date of Issued | 2018-05 |
Rights |
Copyright (c) 2018 Hiroshima University Medical Press
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Publish Type | Version of Record |
Access Rights | open access |
Source Identifier |
[ISSN] 0018-2052
[NCID] AA00664312
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