Liposome collapse resulting from an allosteric interaction between 2,6‐dimethyl‐β‐cyclodextrins and lipids

RSC Advances Volume 5 Issue 95 Page 77746-77754 published_at 2015-09-09
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Title ( eng )
Liposome collapse resulting from an allosteric interaction between 2,6‐dimethyl‐β‐cyclodextrins and lipids
Creator
Iwata Noboru
Hino Shodai
Mae Tomoya
Tsuchiya Yuki
Ohara Kazuaki
Yamaguchi Kentaro
Source Title
RSC Advances
Volume 5
Issue 95
Start Page 77746
End Page 77754
Abstract
Although heptakis(2,6‐di‐O‐methyl)‐β‐cyclodextrin (DMe‐β‐CDx) has been reported to exhibit higher cytotoxicity than many other cyclodextrins because of the way in which it abstracts cholesterols from liposomes, we have identified another reason for its cytotoxicity based on its interaction with lipids. These interactions exhibited nonlinear sigmoidal responses with Hill coefficient values (n) in the range of 3.0‒3.6, which indicated that this phenomenon involves positive allosterism. Furthermore, analysis by mass spectroscopy revealed that the lipid•DMe‐β‐CDx complexes had stoichiometric ratios in the range of 1:1‒1:4.
Descriptions
Electronic supplementary information (ESI) available: Average hydrodynamic diameter values and UV-vis absorption, 1H NMR, NOESY and CSI-MS spectra. See DOI: 10.1039/c5ra14970c
This work was supported by a JSPS KAKENHI a Grant‐in‐Aid for Scientific Research (B) (Grant No. 25288037).
NDC
Chemistry [ 430 ]
Language
eng
Resource Type journal article
Publisher
Royal Society of Chemistry
Date of Issued 2015-09-09
Rights
This journal is © The Royal Society of Chemistry 2015
This is an open access article distributed under the terms of the Creative Commons Attribution 3.0 Unported Licence.
Publish Type Version of Record
Access Rights open access
Source Identifier
[ISSN] 2046-2069
[DOI] 10.1039/c5ra14970c