Ecophysiological Role and Function of Uncultured Chloroflexi in an Anammox Reactor
Water Science and Technology Volume 66 Issue 12
Page 2556-2561
published_at 2012-10
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| File | |
| Title ( eng ) |
Ecophysiological Role and Function of Uncultured Chloroflexi in an Anammox Reactor
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| Creator |
Yuri Shota
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| Source Title |
Water Science and Technology
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| Volume | 66 |
| Issue | 12 |
| Start Page | 2556 |
| End Page | 2561 |
| Abstract |
The coexistence of uncultured heterotrophic bacteria belonging to the phylum Chloroflexi has often been observed in anaerobic ammonium oxidation (anammox) reactors fed with synthetic nutrient medium without organic carbon compounds. To determine if coexisting Chloroflexi in anammox reactors scavenge organic matter derived from anammox bacterial cells, the present study was conducted to investigate the substrate uptake pattern of the uncultured Chloroflexi present in an anammox reactor and to clarify if they take up microbial products derived from anammox bacterial cells. To accomplish this, combined microautoradiography and fluorescence in situ hybridization (MAR-FISH) was conducted. Phylogenetic analysis revealed that 36% of the clones analyzed in this study were affiliated with Chloroflexi. The sequence similarities to Anaerolinea thermophila and Caldilinea aerophila within the phylum Chloroflexi were only 81.0-88.7% and 80.3-83.8%, respectively. The uncultured Chloroflexi were found to incorporate sucrose, glucose, and N-acetyl-glucosamine. The 14C-tracing experiment revealed that the uncultured Chloroflexi were clearly MAR-positive, indicating the utilization of decaying anammox bacterial cell materials. Taken together, these results indicate that coexisting uncultured Chloroflexi in anammox reactors scavenge organic compounds derived from anammox bacterial cells.
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| Keywords |
Anammox
Chloroflexi
MAR-FISH
phylogenetic analysis
substrate uptake pattern
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| Descriptions |
This research was partially supported by an Assistance for Research and Development of Construction Technology grant from the Ministry of Land, Infrastructure, Transport and Tourism, and by a Grant-in-Aid for Young Scientists (B) from the Ministry of Education, Culture, Sports, Science and Technology.
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| NDC |
Construction. Civil engineering [ 510 ]
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| Language |
eng
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| Resource Type | journal article |
| Publisher |
IWA Publishing
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| Date of Issued | 2012-10 |
| Rights |
Copyright (c) IWA Publishing 2012
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| Publish Type | Author’s Original |
| Access Rights | open access |
| Source Identifier |
[ISSN] 0273-1223
[DOI] 10.2166/wst.2012.479
[DOI] http://doi.org/10.2166/wst.2012.479
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