Histochemical studies on the mucin of the chicken salivary glands

鶏の唾液腺ムチンの組織化学

Fujii Shunsaku

Tamura Tatsudo

広島大学水畜産学部紀要

Journal of the Faculty of Fisheries and Animal Husbandry, Hiroshima University

　鶏の口腔に開口するいわゆる唾液腺はいずれも粘液性腺であるといわれている. しかしこの唾液腺粘液物質の性状についての報告は少ない.そこで鶏の唾液線ムチンを組織化学的に観察した.
　調べた9 種の唾液腺はすべて粘液性腺であり，粘液物質は酸性粘液多糖類の性状を示した.しかし腺により多少酸性粘液の性状が異なっていた.詳しくは，単口上顎腺，前舌腺，口角腺のムチンは主として非硫酸性粘液多糖類の性質を示し，口蓋腺，前および後顎下腺，蝶形翼状腺，後舌腺，輪状披裂腺のそれは主として硫酸性粘液多糖類の性質を示した.

The mucin of the salivary glands of the chiken was examined histochemically. The results obtained are as follows.

(1) In all the salivary glands, the contents of the glandular cell and the secretion in the glandular lumen were strongly periodic acid-Schiff reactive, azure A-metachromatic at a low value of pH, and alcianophilic, and showed affinity to various acid mucin stains. Methylation completely deprived the glandular cell of such properties as alcianophilia and metachromasia. Saponification after methylation almost completely restored those properties blocked by methylation. Digestion with such an enzyme as diastase, hyaluronidase, or sialidase failed to modify the staining properties of the glandular cell. The cell was almost negative for ninhydrin-Schiff and mercuric bromphenol blue reactions for protein. From the results of these histochemical reactions, it was concluded that the salivary glands of the chiken were all mucin-secreting ones, and that the mucosubstance contained in the glands was acid mucopolysaccharide in nature.

(2) The acid mucin of the salivary glands was considered as compounds of nonsulfated and sulfated acid mucopolysaccharide. It was different in composition from one gland to another. The salivary glands were divided into two groups on the basis of this difference. The maxillary, anterior lingual, and angulus oris glands were composed mainly of nonsulfated acid mucopolysaccharide and classified into
one group. The palatine, anterior and posterior submandibular, spheno-pterygoid, posterior lingual, and crico-arytenoid glands consisted mainly of sulfated acid mucopolysaccharide and formed the other group.

動物学 [ 480 ]

英語

広島大学水畜産学部

[ISSN] 0440-8756

[NCID] AN00213563