Effect of Filter on Specific (3H)-5-Hydroxytryptamine Binding to Synaptic Membranes
Hiroshima Journal of Medical Sciences Volume 31 Issue 2
Page 129-131
published_at 1982-06-10
アクセス数 : 970 件
ダウンロード数 : 141 件
今月のアクセス数 : 1 件
今月のダウンロード数 : 0 件
この文献の参照には次のURLをご利用ください : https://ir.lib.hiroshima-u.ac.jp/00038386
| File | |
| Title ( eng ) |
Effect of Filter on Specific (3H)-5-Hydroxytryptamine Binding to Synaptic Membranes
|
| Title ( jpn ) |
シナプス膜への特異的セロトニン結合に及ぼすフィルターの影響
|
| Creator |
Maeoka Kunihiko
Sarai Keisuke
|
| Source Title |
Hiroshima Journal of Medical Sciences
|
| Volume | 31 |
| Issue | 2 |
| Start Page | 129 |
| End Page | 131 |
| Journal Identifire |
[PISSN] 0018-2052
[EISSN] 2433-7668
[NCID] AA00664312
|
| Abstract |
In the present study, the authors carried out some basic examinations of specific (3H)-5-Hydroxytryptamine (5-HT, serotonin) receptor binding assay. The most adequate incubation condition for (3H)-5-HT binding assay was at 0°C for 10 to 30 min. Scatchard plot of (3H)-5-HT binding using a Millpore filter and a Glass filter drew biphasic and monophasic, respectively. It is suggested that filter may affect on specific (3H)-5-HT binding. Therefore, the characteristics of filter should be taken into consideration in (3H)-5-HT binding assay.
|
| Keywords |
5-HT receptor binding assay
Filter
Low-affinity
High-affinity
|
| NDC |
Medical sciences [ 490 ]
|
| Language |
eng
|
| Resource Type | departmental bulletin paper |
| Publisher |
Hiroshima University School of Medicine
|
| Date of Issued | 1982-06-10 |
| Publish Type | Version of Record |
| Access Rights | open access |
| Source Identifier |
[ISSN] 0018-2052
[NCID] AA00664312
[PMID] 7129950
|
